Study of Cryopreservation in Rapeseed (Brassica napus L.) Seeds by Vitrification Method

Document Type : research

Authors

Abstract

In this experiment, cryopreservation of rapeseed seeds (Milena and ARC-2 cultivars ) via vitrification method was carried out using two protective solutions, PVS2 and PVS3 at 5 treatment times (20, 40, 60, 80 and 100 min). The experiment was conducted as a factorial based on completely Randomized Design with three replications. The results showed significant differences (p<0.01) between different treatment times, genotypes and interactions of these two factor for germination percentage. Also, interactions of treatment times and protective solutions were significant (p<0.05) for germination percentage. Treatment of rapeseed seeds (Milena cultivar) with protective solution for 100 min showed the highest germination compared with to other treatments. Also, treatment of rapeseed seeds with PVS2 solution for 100 min and with PVS3 for 20 and 60 min showed the highest germination percentage, respectively, compared to other treatment. Concerning the trait of root length, the treatment time and genotype and their interaction showed a significant differences (p<0.01). The interaction of genotype and protective solution and also triple interaction of three factors showed significant effect (p< 0.05) on root length of rapeseed seeds. So, treatment of rapeseed seeds with PVS3 solution for 20 min produced the highest mean for root length. Different times of treatment and also, the triple interactions of genotype, kind of solution and time of treatment showed significant effects (p<0.01) on shoot length of rapeseed seeds. Treatment of rapeseed seeds (cultivar of ARC-2) with PVS3 solution created the highest shoot length compared to other treatments.

Keywords

References

Ashmore, S. E. 1997. Status report on the development and application of in vitro technique for the conservation and use of plant genetic resources.IPGRI, Rome, 67pp.
Belletti, P., Lanteri, S., Lepori, G., Nassi, M. O. and  Quagliotti, L. 1990. Factors related to the cryopreservation of pepperand egg plant seeds. Advanced Horticulture Science, 4: 118-120.
Benson, E., Wilkinson, M., Todd, A., Ekuere, V. and  Lyon, I.  1996. Development competence and ploidy stability in plants regenerated from cryopreserved potato shoot tips. Cryo-Letters,17:119-128.
Benzing, D. H.  2000. Bromeliaceae: profile of an adaptive radiation. New York, Cambridge University Press.
 Chmielarz, P. 2010. Cryopreservation of the non-dormant orthodox seeds of Ulmus glabra. Acta Biologica Hungarica, 61: 224-233.
Gonçalves, S., Fernandes, L., Pérez-García, F., González-Benito, M. E. and Romano, A. 2009. Germination requirements and cryopreservation tolerance of seeds of the endangered species Tuberaria major. Seed Science and Technology, 37: 480-484.
Kakaei, M. 2009. Effects of genotype and drought stress on physiological, morphological, phonological and biochemical traits in winter rape (Brassica napus L.). M. Sc. Thesis, Islamic azad university, kermansha - Iran.
Kholina, A. B. and Voronkova,  N. M. 2012. Seed cryopreservation of some medicinal legumes. Journal of Botany, 10: 165-172.
Li, Y., Qu, J., Dong, Z., Wang, T. and An, L. 2008. Storage behavior of Zygophyllum xanthoxylon (Bge.) Maxim seeds at low moisture contents. Acta Physiologiae Plantarum, 30: 651-656.
Lynch, P. T., Benson, E. E. and Harding, K. 2007. Invited commentary climate change: the role of ex situ and cryo-conservation in the future security of economi­cally important, vegetatively propagated plants. Journal of Horticultural Science and Biotechnology,82: 157-160.
Martinkova, Z. and Honěk, A. 2007. The effect of cryopreservation on germination of dandelion seeds. Plant Protection Science, 43:63-67.
Moukadiri, O., Deming, J., O’Connor, J. B. and Cornyo, M. J. 1999. Phenotypic characterization of the progenies of rice plants derived from cryopreserved calli. Plant Cell Report, 18: 625-632.
Sakai, A., Kobayashi, S. and Oiyama, I. 1990. Cryopreservation of nuclear cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Report, 9: 30-33.
Towill, L. E. 1991. Cryopreservation In Dodds J. H. (eds), In vitro methods for conservation of plant genetic resources. Chapman and Hall, London, 41-70 pp.
Volk, G. M., Harris, J. L. and Rotindo, K. E. 2006. Survival of mint shoot tips after exposure to cryoprotectant solution components. Cryobiology, 52: 305-308.
Wakui, K., Sato, C., Takahata, Y. and Kaizuma, N. 1998. Long-term storage and cryopreservation of desiccated microspore-derived embryos in Brassica spp. Plant Biotechnology, 15: 123-126.

Files

Share

How to cite

Statistics