شناسایی باکتری‌های تجزیه‌کننده نفت از مناطق آلوده پلدختر و بررسی فاکتورهای مؤثر بر کارآیی تجزیه در آنها

نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشجوی کارشناسی‌ارشد بیماری‌شناسی گیاهی، دانشکده کشاورزی دانشگاه لرستان، لرستان

2 استادیار گروه گیاهپزشکی، دانشکده کشاورزی دانشگاه لرستان، لرستان

3 مربی گروه گیاهپزشکی، دانشکده کشاورزی دانشگاه لرستان، لرستان

چکیده

    آلودگی اکوسیستم‌های طبیعی توسط آلاینده‌های نفتی به‌دلیل پایداری بالا و اثرات زیان‌بار بر محیط‌زیست و سلامت انسان­ها بیشتر مورد توجه قرار گرفته است. امروزه از روش­های متعدد فیزیکی، شیمیایی، حرارتی و زیست پالایی به‌منظور اصلاح محیط­های آلوده استفاده می­شود. فناوری‌ زیست پالایی بر پایه‌ی استفاده از ریزجانداران طبیعی و یا تراریخت به‌‌منظور احیای مجدد مکان‌های آلوده و حفاظت از محیط‌‌زیست استوار است. در این پژوهش نمونه­برداری از خاک و آب آلوده منطقه تنگ­فنی پلدختر به‌منظور جداسازی و شناسایی باکتری‌های بومی تجزیه‌کننده ترکیبات نفتی صورت گرفت. نمونه­ها پس از انتقال به آزمایشگاه روی محیط­کشت­های نوترینت آگار، نوترینت براث و 2xYT کشت گردیدند. بعد از خالص‌سازی، جدایه­های باکتریایی براساس خصوصیات فنوتیپی - بیوشیمیایی و توالی­یابی بخشی از ناحیه ژن 16SrDNA به‌عنوان گونه­های Pseudomonas aeruginosa، Acinetobacter junii، Acinetobacter baumannii،Delftia tsuruhatensis،Sphingobacterium multivorum، Stenotrophomonas acidaminiphila، Comamonas koreensis شناسایی شدند. همچنین جدایه­های باکتریایی از نظر فاکتورهای تشکیل بیوفیلم، تولید سیدروفور و حرکت اسوارمینگ مورد بررسی قرار گرفتند، که بیشترین میزان تشکیل بیوفیلم و حرکت اسوارمینگ مربوط به گونه Pseudomonas aeruginosaبود. در این تحقیق برای نخستین بار باکتریComamonas koreensis به‌عنوان تجزیه‌کننده نفت معرفی گردید.
 

کلیدواژه‌ها

عنوان مقاله [English]

Identification of Oil Degrading Bacteria from Poldokhtar Polluted Areas and Investigation of Factors Affecting Their Degradation Performance

نویسندگان [English]

  • soma Narimany 1
  • Eidi Bazgir 2
  • Hosein Mirzaee Najafgholi 3
1 Plant Pathologists Graduate Student, Department of Plant Protection,Faculty of Agriculture, University of Lorestan, Khoramabad
2 Assistant Professor, Department of Plant Protection, Faculty of Agriculture, University of Lorestan, Khoramabad
3 Coach, Department of Plant Protection, Faculty of Agriculture, University of Lorestan, Khoramabad
چکیده [English]

Pollution of natural ecosystems by petroleum compounds have been more considered because of their high persistence in environment and adverse impacts on human health. Nowadays, a wide range of physical, chemical, thermal and bioremediation methods are employed in order to remediate contaminated sites. Bioremediation technology is based on use the natural or engineered microorganisms for reclamation of contaminated sites and environmental conservation. The current study was carried out in order to isolate and characterize the oil degrading native bacteria from Tange Fani Poldokhtar region, Lorestan province, Iran. Soil and water of contaminated sites were sampled and after transfer to the laboratory, samples were cultivated on the nutrient agar, nutrient broth and 2xYP media. After purification, bacterial strains were identified on the basis of phenotypic-biochemical traits and sequence of 16S rDNA gene as Pseudomonas aeruginosa, Acinetobacter junii, Acinetobacter baumannii, Delftia tsuruhatensis, Sphingobacterium multivorum, Stenotrophomonas acidaminiphila and Comamonas koreensis. Furthermore, the bacterial strains were evaluated regarding biofilm formation, siderophore production and swarming motion, which the highest amount of biofilm formation and swarming motion was belonged to P. aeruginosa. In this study, bacterium Comamonas koreensis for the first time as oil degrading introduced.

کلیدواژه‌ها [English]

  • Petroleum compounds
  • Bioremediation
  • Bacteria
  • Pseudomonas aeruginosa
  • Biofilm

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