بررسی تغییرات ناشی از جهش با اتیل‌متان‌سولفونات (EMS) در لاین‌های موتانت برنج با نشانگر ISSR

نوع مقاله : علمی - پژوهشی

نویسندگان

1 استادیار گروه بیوتکنولوژی، دانشکده مهندسی انرژی و فناوری های نوین، دانشگاه شهید بهشتی تهران

2 دانش‌آموخته کارشناسی ارشد، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده تولید گیاهی دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان

3 دانشیار گروه اصلاح نباتات و بیوتکنولوژی، دانشکده تولید گیاهی دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان

4 دانش‌آموخته کارشناسی‌ارشد، گروه زراعت و اصلاح نباتات، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری

چکیده

    استفاده از جهش جهت ایجاد تنوع ژنتیکی و مواد ژنتیکی جدید از دیرباز متداول بوده و از آن برای بهبود خواص کمّی و کیفی مختلف در گیاهان سود جسته‌اند. در این تحقیق، هجده لاین موتانت برنج که برای پاکوتاهی، تعداد پنجه زیاد، زودرسی و عملکرد بالا از بین یک جمعیت موتانت حاصل از جهش‌زایی رقم الیت ندا با اتیل‌متان‌سولفونات (EMS) انتخاب شده بودند، مورد ارزیابی مولکولی با آغازگرهای ISSR قرار گرفتند. از 10 آغازگر مورد استفاده، 8 آغازگر DNA ژنوتیپ‌های مختلف را تکثیر کردند. تعداد کل نوارهای تولید شده 67 نوار (به ازای هر آغازگر به‌طور متوسط 4/8 نوار) بود که 7/53 درصد آن‌ها چندشکلی بین ژنوتیپ‌ها را نشان دادند. متوسط ضریب تنوع ژنی نئی در جمعیت مورد مطالعه، 1/14 درصد به‌دست آمد. بیشترین شباهت به رقم مادری (8/86 درصد) مربوط به گروه موتانت‌های پرعملکرد و کمترین شباهت (7/76 درصد) مربوط به گروه موتانت‌های پرپنجه بود. تجزیه خوشه‌ای با روش UPGMA، رقم مادری را در یک گروه و 4 دسته لاین‌های موتانت را در گروه جداگانه‌ای قرار داد. تجزیه خوشه‌ای در هر گروه از لاین‌های موتانت نیز به‌طور جداگانه انجام شد که به استثنای دسته موتانت‌های انتخابی برای تعداد پنجه زیاد، در هر سه دسته دیگر رقم مادری به‌تنهایی در یک گروه و همه موتانت‌های انتخابی در گروه جداگانه‌ای قرار گرفتند. براساس نتایج این تحقیق، می‌توان نتیجه‌گیری کرد که تکنیک جهش برای ایجاد تنوع ژنتیکی در برنج مطلوب بوده و از نشانگرهای ISSR می­توان برای شناسایی لاین‌های موتانت در جمعیت‌های حاصل از جهش‌زایی با EMS استفاده کرد.

کلیدواژه‌ها

عنوان مقاله [English]

Assessment of EMS-induced Variations In rice Mutant Lines Using ISSR Marker

نویسندگان [English]

  • Asadollah Ahmadikhah 1
  • Hoda Shojaeian 2
  • M.Hadi Pahlevani 3
  • Leila Nayyeripasand 4
1 Assistant Proffesor, Department of Biotechnology, Faculty of Energy Engineering and Modern Technologies, Shahid Beheshti University, Tehran
2 Former M.Sc. Student, Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan
3 Associate Professor, Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan
4 Former Msc. Student, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Sari University of Agricultural Sciences and Natural Resources, Sari
چکیده [English]

Abstract
Using mutation has been relevant to induce genetic variation and new genetic material, and it was used to improve different qualitative and quantitative traits in crop plants. In this research, eighteen rice mutant lines which were selected from a mutant population developed from mutagenesis of rice elite cultivar Neda by using ethyl methane sulfonate (EMS) for reduced plant height, higher tiller number, early maturation and higher yield, were evaluated at molecular level using ISSR markers. Eight out of 10 primers successfully amplified DNA from different genotypes. In total, 67 bands (in average 8.4 bands per primer) were produced, 53.7% of which showed polymorphism between genotypes. Average Nei`s genetic diversity in the studied population was 14.1%. Highest similarity to original cultivar (86.8%) belonged to high-yielding mutant group and lowest similarity (76.7%) belonged to high-tiller mutant group. Cluster analysis using UPGMA method, placed the original cultivar alone in one cluster and 4 mutant groups in another distinct cluster. Cluster analysis was also performed separately in each sub-cluster that except for high-tiller group it could discriminate the original cultivar from any other three mutant groups. On the basis of the results of this research, it can be concluded that mutation technique was a desirable method for inducing genetic variation in rice and also it is possible to use ISSR markers for identification of mutant lines in populations developed from mutagenesis by EMS.
 

کلیدواژه‌ها [English]

  • EMS
  • Molecular marker
  • Mutant
  • Rice

اصل مقاله

 

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