شناسایی QTLs مرتبط با برخی از صفات مورفولوژیکی در توتون شرقی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد گروه اصلاح و بیوتکنولوژی گیاهی، دانشکده کشاورزی دانشگاه ارومیه، ارومیه

2 دانشیار گروه اصلاح و بیوتکنولوژی گیاهی، دانشکده کشاورزی دانشگاه ارومیه، ارومیه

3 استادیار گروه زراعت و اصلاح نباتات، دانشکده کشاورزی دانشگاه مراغه، مراغه

چکیده

به منظور شناسایی مکان­های ژنی مرتبط با صفات مورفولوژیک شامل تعداد برگ، طول برگ، عرض برگ، سطح برگ، وزن تر برگ، وزن خشک برگ، ارتفاع گیاه و قطر ساقه در توتون ‌شرقی، یک جمعیت ژنتیکی متشکل از ۱۰۰ بوته ۲F حاصل از تلاقی دو ژنوتیپ توتون شرقی ۴۰۶ SPT (والد پدری) و ۳۱ Basma seres (والد مادری)، مورد ارزیابی قرار گرفت. برای شناسایی QTLها، نقشه پیوستگی با ۲۳ نشانگر SSR و ۲۹ نشانگرISSR  تهیه گردید که 8/570 سانتی­مورگان از ژنوم توتون را پوشش می­دهد. نتایج مکان­یابی ژنی نشان داد که ۱۱ QTL در کنترل صفات مورد مطالعه نقش دارند. حداکثر تغییرات فنوتیپی توجیه شده توسط QTLها 2/25 درصد بود. در این بررسی، برای صفت تعداد برگ هیچ QTLی شناسایی نگردید. نتایج نشان داد که برخی QTLهای شناسایی شده برای صفات ارتفاع گیاه، طول برگ، قطر ساقه، وزن خشک و تر برگ هم مکان هستند که می‌توانند باعث افزایش کارآیی انتخاب به کمک نشانگر و پیشبرد برنامه‌های به‌نژادی گیاهی در توتون شوند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Identification of QTLs Associated with Some Morphological Traits in Oriental Tobacco

نویسندگان [English]

  • Faramarz Hoshyardel 1
  • Reza Darvishzadeh 2
  • Hamid Hatami Maleki 3
1 Urmia University
3 University of Maragheh
چکیده [English]

In order to identify quantitaive trait loci (QTLs) associated with morphological traits including number of leaves, leaf length, leaf width, leaf area, leaf fresh weight, leaf dry weight, plant height and stem diameter in oriental type tobacco, a genetic population comprising 100 F2 individuals from a cross between two oriental type genotypes, namely Basma seres 31 (maternal) x SPT 406 (paternal) was evaluated under field conditions. Subsequently a linkage map  based on 23 simple sequence repeat (SSR) markers and 29 inter simple sequence repeat (ISSR) markers was constructed which covered 570.8 cM of tobacco genome. Composite interval mapping could identify 11 QTLs involved in controlling the investigated traits. The maximum percentage of phenotypic variance (R2) explained by QTLs was 25.2%. In this study, no QTL was identified for number of leaves. The results showed the existence of co-localized QTLs for some of the studied traits including plant height, leaf length, stem diameter and leaf dry weight which enhances the efficiency of marker-assisted selection in tobacco breeding programs.

کلیدواژه‌ها [English]

  • Gene mapping
  • ISSR marker
  • quantitative traits
  • SSR marker
  • transgressive segregation

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