محتوی سولفارافان در اندام‌های مختلف گیاه Lepidium draba در اکوتیپ‌های مختلف استان کرمان

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناس ارشد گروه بیوتکنولوژی، دانشکده علوم و فناوری‌های نوین، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان، ایران.

2 استادیار گروه بیوتکنولوژی، پژوهشکده علوم محیطی، پژوهشگاه علوم و تکنولوژی پیشرفته و علوم محیطی، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان

3 کارشناس ارشد گروه بیوتکنولوژی، دانشکده علوم و فناوری‌های نوین، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان

چکیده

ازمک (Lepidium draba)، یک علف هرز چندساله از خانواده براسیکاسه است که حاوی مقدار قابل‌توجهی گلوکورافانین از گروه گلوکوزینولات­ها می­باشد. این متابولیت تحت هیدرولیز آنزیمی میروزیناز، نوعی ایزوتیوسیانات به‌نام سولفارافان تولید می­نماید که خواص ضدسرطانی متعددی دارد. مشخص شده است که عوامل محیطی مختلف از قبیل دما و ارتفاع بر بیوسنتز متابولیت­های ثانویه تأثیر دارند. در این مطالعه، محتوی سولفارافان در بخش­های مختلف گیاه بالغ L. draba (شامل ریشه، برگ، گل و میوه) جمع­آوری شده از اکوتیپ­های مختلف استان کرمان (ماهان، بافت و جیرفت (ساردوئیه)) با استفاده از دستگاه کروماتوگرافی مایع با کارایی بالا اندازه­گیری شد. نتایج نشان‌دهنده میزان بالای این ایزوتیوسیانات در میوه این گیاه می­باشد که در مرتبه­های بعدی، گل، برگ و ریشه‌های قرار دارند. همچنین محتوی سولفارافان در تمامی اندام­ها، در گیاهان جمع­آوری شده از شهر ماهان در مقایسه با دو شهر دیگر بیشتر بود. با توجه به ارتفاع  کمتر  و درجه حرارت بالاتر شهر ماهان نسبت به دو شهر دیگر استنباط می­شود که این عوامل تأثیر بسزایی در بیوسنتز این ماده مؤثره دارند.

کلیدواژه‌ها


عنوان مقاله [English]

Sulforaphane Content in Different Organs of Lepidium draba from Different Ecotype of Kerman Province

نویسندگان [English]

  • Maryam Aminizadeh 1
  • Ali Riahi-Madvar 2
  • Mehdi Mohammadi 3
چکیده [English]

Lepidium draba L. is a perennial weed belongs to the Brassicaceae family, contains considerably amount of glucoraphanin from glucosinolates group. Hydrolysis of this metabolite via myrosinase activity produce an isothiocyanate named sulforaphane that has various anticancer properties. It has been shown that environmental conditions such as temperature and altitude affect the biosynthesis level of secondary metabolites. In the present study, mature plants of L. draba was collected from different ecotypes of Kerman province [Mahan, Baft, Jiroft (Sardooiyeh)] and then sulforaphane content of each parts (root, leaf, flower and fruit) was measured using High Performance Liquid Chromatography. The results showed that fruits have the maximum amount of this compound, followed by flowers, leaves and roots. Also, the sulforaphane content in all organs of the plants where collected from Mahan was higher than two other cities. Based on the higher temperature and lower altitude of Mahan compared to the other cities, it deduced that these factors have significant effects on biosynthesis level of this active ingredient.

کلیدواژه‌ها [English]

  • Isothiocyanate
  • Glucosinolate
  • HPLC
  • Myrosinase
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