آنالیز ترانسکریپتوم ژن‌های القاء شده در پاسخ به تنش شوری در گیاه شورپسند Aeluropus littoralis

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناس‌ارشد اصلاح نباتات، پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری،

2 استاد پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری، مازندران

3 استادیار گروه بیوتکنولوژی کشاورزی، دانشکده مهندسی انرژی و فناوری‌های نوین،دانشگاه شهید بهشتی، تهران

4 دانشجوی دکتری اصلاح نباتات، پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری، مازندران

چکیده

شوری خاک با محدود کردن تولیدات گیاهی ابتدا جنبه‌های نموی را تحت تأثیر قرار می‌دهد که منجر به کاهش تولیدات گیاهی می‌گردد. در این تحقیق آنالیز بیان ژن مبتنی بر تکنیک cDNA-AFLP جهت مقایسه الگوی بیانی ناشی از تنش شوری در سه سطح 0 (شاهد)، 200 و 400 میلی‌مولار NaCl به‌مدت 1 هفته پس از واکشت، در ریشه گیاه Aleuropus littoralis که نزدیک­ترین خانواده به غلات می­باشد، انجام شد. از میان 32 عدد EST  جداسازی شده از روی ژل پلی‌اکریل‌آمید، 25 عدد با طول میانگین 250 جفت باز توالی‌یابی شدند. به‌میزان 80% از رونوشت­های توالی‌یابی شده با توالی اسیدآمینه و اسیدنوکلئیک شناسایی شده در موجودات دیگر، شباهت نشان دادند. از طرفی دیگر، 7 رونوشت (EST) به‌عنوان ژن­های جدید احتمالی در نظر گرفته شدند. در نهایت 25 عدد EST  در بانک ژن به ثبت رسید که مهم‌ترین آنها شامل گروه­های پروتئینی انتقال‌دهنده پتاسیم، پروتئین­های ریبوزومی، NADH­ دهیدروژناز و گولگین می­باشند. نتایج این تحقیق در درک پایه­های مولکولی تنش شوری و مکانیسم مقاومت جهت اصلاح و مهندسی ژنتیک در بهبود مقاومت غلات در برابر این تنش و تولید گیاهان مقاوم کمک می‌کند.
 

کلیدواژه‌ها

عنوان مقاله [English]

Transcriptom Analysis of Induced Genes in Response to Salt Stress in the Halophyte Aeluropus littoralis

نویسندگان [English]

  • farzaneh fatemi 1
  • ghorbanali nematzadeh 2
  • hossein askari 3
  • seyedhamidreza hashemi 4
1 MSc of Plant Breeding, Genetic and Agricultural Biotechnology Institute of Tabarestan, University of Agriculture and Natural Resources of Sari
2 Prophosser of Genetic and Agricultural Biotechnology Institute of Tabarestan, University of Agriculture and Natural Resources of Sari
3 Professor assistant of Department of Biotechnology, Faculty of New Technologies and Energy Engineering, Shahid Beheshti University, Tehran
4 Genetic and Agricultural Biotechnology Institute of Tabarestan, University of Agriculture and Natural Resources of Sari
چکیده [English]

Soil salinity limits crop production and affects developmental aspects at first Leading to reduced plant production. In this study, gene expression analysis based on cDNA-AFLP technique was used to compare the expression profiles of NaCl stress at three levels, 1 week after subculture: 0 (control), 200mM and 400mM, in roots of Aeluropus littoralis which is the closest family to cereal. Among 32 isolated ESTs, 25 ESTs were obtained with the average length of 250 bp. The nucleotide sequences were compared with those in the GenBank database. Approximately 80% of the ESTs show homology to nucleotide or amino acid sequences in the GenBank database and 7 ESTs show no significant similarity in the GenBank database which considered as novel genes. Finally, 25 ESTs were recorded in NCBI database which are included potassium transporter, ribosomal protein, NADH dehydrogenase and golgin. The result of this research is very important to understand molecular basis and resistance mechanisms of drought stress for breeding and genetic engineering to improve crop resistance against stress and the production of resistant plants.

کلیدواژه‌ها [English]

  • Salinity
  • cDNA-AFLP
  • Gene expression
  • Aeluropus littoralis

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