مقایسه کارآیی نشانگرهای مولکولی SSR و RAPD در برنامه گزینش به کمک نشانگر (MAS) در برنج

نوع مقاله: علمی - پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی‌ارشد بیوتکنولوژی کشاورزی، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان

2 دانشیار گروه بیوتکنولوژی، دانشکده مهندسی و فناوری‌های نوین، دانشگاه شهید بهشتی تهران، تهران

3 دانشیار گروه اصلاح نباتات و بیوتکنولوژی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان

چکیده

گزارش­های متعددی از مقایسه کارآیی نشانگرهای مولکولی برای برآورد پارامترهای ژنتیکی گیاهان در مطالعات تنوع ژنتیکی منتشر شده است. اغلب تحقیقات بر روی ژنوم­های گیاهی مختلف و برنامه­های اصلاحی گوناگونی انجام گرفته است، ولی در این میان گزارشی مبنی بر مقایسه کارآیی نشانگرهای SSR و RAPD در برنامه­های گزینش به کمک نشانگر (MAS) در ژنوم برنج مشاهده نشده است. به همین منظور، در این تحقیق کارآیی دو سیستم نشانگری فوق جهت تخمین برخی پارامترهای ژنتیکی در لاین­های تلاقی برگشتی پیشرفته در برنج مورد مقایسه قرار گرفت. از 100 جفت آغازگر SSR و20 آغازگر RAPD برای انگشت­نگاری ژنوتیپ­ها استفاده شد. چندشکلی نشانگرهای RAPD بیشتر از نشانگرهای SSR، اما میزان اطلاعات چندشکلی نشانگر SSR بیش از دو برابر نشانگر RAPD به‌دست آمد. همبستگی ضعیفی میان دو سیستم مختلف نشانگری برای تخمین شباهت ژنتیکی نتاج به والد تکراری مشاهده گردید. همچنین نتایج نشان داد که افزایش تعداد نشانگر RAPD تأثیری در افزایش دقت برآوردها نداشت. با توجه نتایج این تحقیق پیشنهاد می­شود که از نشانگر SSR در برنامه­های گزینش استفاده شود.

کلیدواژه‌ها


عنوان مقاله [English]

Comparison of the Efficiency of SSR and RAPD Markers in Marker-Assisted Selection (MAS) Program in Rice

نویسندگان [English]

  • Mahdi Mirarab 1
  • Asadollah Ahmadikhah 2
  • Mohammad Hadi Pahlevani 3
1 M.Sc. Graduate, Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan
2 Associate Professor, Department of Biotechnology, Faculty of Engineering and Modern Technologies, Shahid Beheshti University, Tehran
3 Associate Professor, Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan
چکیده [English]

Several reports were published in regard to the comparison of the efficiency of different molecular markers in genetic diversity studies for estimating genetic parameters. Most researches have been conducted out in different plant genomes and in different breeding programs, but there isn`t any report on comparing the efficiency of SSR and RAPD markers in marker-assisted selection (MAS) programs in rice genome. In this line, a research was conducted out to compare the two marker systems for estimating some genetic parameters in advanced backcross lines in rice. One hundred SSR primer pairs and 20 RAPD primers were used for fingerprinting of the studied genotypes. Polymorphism of RAPD markers was higher than SSR markers, while polymorphism information content (PIC) of SSR markers was more than 2 fold relative to RAPD markers. A weak correlation was observed between the two systems for estimating genetic similarity of progenies to recurrent parent. Also, results showed that increasing the number of RAPD markers didn`t cause any increase in estimation precision. The results of this research suggested the use of SSR markers in selection programs. 

کلیدواژه‌ها [English]

  • Rice
  • Marker-assisted selection
  • SSR
  • RAPD
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