تنوع ژنتیکی و صفات فیزیولوژیکی استرین‌های Ralstonia solanacearum مزارع سیب زمینی استان همدان

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی‌ارشد بیماری‌شناسی گیاهی، دانشکده کشاورزی، دانشگاه بوعلی‌سینا، همدان

2 استاد بیماری‌شناسی گیاهی، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه بوعلی‌سینا، همدان

چکیده

بیماری پژمردگی باکتریایی ناشی از Ralstonia solanacearum از بیماری­های مهم سیب­زمینی در استان همدان است. برای بررسی تنوع ژنتیکی جدایه‌های R. solanacearum نمونه‌های گیاهی مشکوک به آلودگی در بهار و تابستان سال 1389 از مناطق مختلف این استان جمع‌آوری گردید. کشت اندام‌های آلوده گیاهی (غده و ساقه) روی محیط نوترینت آگار (NA) حاوی تری فنیل تترازولیوم کلراید و بررسی خصوصیات بیوشیمیایی و فیزیولوژیکی، منجر به شناسایی 52 استرین R. solanacearum بیوار A2 گردید. برای تشخیص دقیق­تر جدایه­ها واکنش زنجیره­ای پلیمراز با استفاده از آغازگرهای ps96H و ps96I انجام شد. نتایج PCR نشان داد که نمونه­ها همراه با نمونه استاندارد یک باند مشابه 148جفت بازی تولید نمودند. تنوع ژنتیکی 26 جدایه انتخابی با آغازگرهای BOX و ERIC مورد بررسی قرار گرفت. طول قطعات تکثیری با استفاده از آغازگر BOX A1R در محدوده 100 تا bp1500 و آغازگر ERIC از200 تا bp1200 متغیر بود. گروه­بندی جدایه­های به‌دست آمده با روش UPGMA و براساس ضریب جاکارد با استفاده از داده­های هر دو آغازگـر، جـدایه­ها را در سطح تشابه 74 درصـد به 5 گروه اصـلی منتسب کرد. نتایج حاصل نشان‌دهـنده‌ی آن است که استرین­های R. solanacearum جمع­آوری شده خصوصیات فنوتیپی و ژنتیکی متفاوتی داشتند، هرچند ارتباط مشخصی بین منطقه جغرافیایی و خصوصیات استرین­ها به‌دست نیامد. این بررسی کارآیی روشPCR  با آغازگرهایBOX  و ERIC را برای نشان دادن چندشکلی ژنتیکی در جمعیت­های R. solanacearum نشان داد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Diversity and Physiological Characteristics of Ralstonia solanacearum from Potato Fields in Hamedan Province

نویسندگان [English]

  • Somayeh Esfandiari 1
  • Gholam Khodakaramian 2
1 M. Sc. Former Student of Plant Pathology, Department of Plant Protection, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan
2 Professor of Plant Pathology, Department of Plant Protection, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan
چکیده [English]

Bacterial wilt disease caused by Ralstonia solanacearum is an important disease on potato in Hamedan province. During spring and summer 2011 infected plants were collected from potato fields to investigate the genetic diversity among R. solanacearum strains. A total of 52 strains were isolated on NA medium containing triphenyl tetrazolium chloride (TTC) from tubers and stems and their phenotypic characteristics revealed that they were belong to biovar 2A. PCR using ps96h and ps96i primers produced 148 bp bands in tested and standard strains. Genetic diversity of 26 selected strains were analyzed by PCR fingerprinting using, ERIC and BOX primers. The length of produced fragments ranged from 200bp to 1200bp in BOX primer and 200bp to 1500bp in rep primer. Tested bacterial strains were grouped based on the obtained molecular DATA from PCR using UPGMA method and Jaccard`s coefficient. Results showed five distinguished clusters. R. solanacearum strains were genetically divers although no direct connection was observed between geographic locations and PCR groups. The results of this study indicated that rep-PCR technique with two ERIC and BOX primers is capable to show genetic polymorphism among different populations of R. solanacearum.

کلیدواژه‌ها [English]

  • Biovar 2A
  • BOX
  • ERIC
  • Potato wilt disease
  • Hamedan province

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دوفصلنامه فن آوری زیستی در کشاورزی(علمی-پژوهشی)
دوره 6، شماره 2
آذر 1394
صفحه 23-31

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