اثر کود نیتروکسین بر میزان بیان ژن Chitinase و Cupi4 در گیاهچه‌های خیار آلوده به Pythium aphanidermatum

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانش آموخته کارشناسی‌ارشد بیماری‌شناسی گیاهی، دانشکده کشاورزی دانشگاه زابل، زابل

2 دانشیار گروه زیست‌شناسی، دانشکده علوم دانشگاه یزد، یزد

چکیده

خیار میزبان بسیاری از عوامل بیماریزای خاکزاد می‌باشد. شبه­قارچ Pythium apanidermatumبه­عنوان یک عامل خاکزاد، سبب بیماری بوته­میری در خیار می‌گردد. با توجه به توسعه کشاورزی پایدار، استفاده از کودهای بیولوژیک برای مدیریت بیماری­های گیاهی امری ضروری خواهد بود. در این تحقیق اثر کود نیتروکسین در مقاومت گیاهچه­های خیار (رقم ES-2862) مورد بررسی قرار گرفت. بعد از تیمار گیاهچه­های آلوده با نیتروکسین، شدت بیماری در مقایسه با شاهد اندازه­گیری شد. از برگ گیاهچه­ها برای استخراج RNA کل و بررسی بیان کمی دو ژن مسئول مقاومت در گیاه با PCR زمان واقعی استفاده شد. نتایج این ارزیابی نشان داد که شدت بیماری در اثر اعمال کود نیتروکسین کاهش یافت. همچنین بیان ژن­های Chitinase و Cupi4در زمان 24 و 48 ساعت بعد از تلقیح افزایش یافت. میزان بیان ژن Cupi4 نسبت به ژن Chitinase تغییراتبیشتری داشت. نتایج این تحقیق نشان می­دهد که کاربرد نیتروکسین می­تواند باعث افزایش مقاومت خیار علیه بیماری مرگ گیاهچه شود.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of Nitroxin Fertilizers on Expression Chitinase and Cupi4 Genes in Cucumber Seedlings Infected to Pythium aphanidermatum

نویسندگان [English]

  • Shahoo Valizadeh 1
  • Seyed Kazem Sabbagh 2
1 M.Sc. Graduated of Plant Pathology, Department of Plant Protection, Faculty of Agriculture, University of Zabol, Zabol
2 Associate Professor, Department of Biology, Faculty of Science, Yazd University, Yazd
چکیده [English]

Pythium apanidermatum is the causal agent of cucumber damping-off disease. Regarding to development of sustainable agriculture, use of bio-fertilizers will be essential for plant disease management. In this study, the effect of nitroxin bio-fertilizer on resistance of cucumber seedlings (ES-2862 cultivar) to damping off disease was investigated. After treatment of infected seedlings with nitroxin, disease severity was measured in comparison with control. The leaves of seedling were used to total RNA extraction and gene expression analysis using Real Time PCR. The results of this assay showed that nitroxin bio-fertilizer reduced the disease severity. Also, expression of Chitinase and Cupi4 genes at 24 and 48 h after inoculation were increased. The the expression of Cupi4 were changed more than that of Chitinase gene. The results of this research indicate that application of nitroxin could increase resistance of sensitive cultivars against damping-off disease.

کلیدواژه‌ها [English]

  • Biofertilizer
  • Nitroxin
  • Gene expression
  • Cucumber
  • Real Time PCR
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