بهینه‌سازی تکثیر درون‌شیشه‌ای آلسترومریا با استفاده از کشت جوانه انتهایی، القاء کالوس و باززایی گیاه

نوع مقاله: علمی - پژوهشی

نویسندگان

1 دانشجوی دکتری گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه گیلان، رشت

2 استادیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه کردستان، سنندج

3 دانشیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان

چکیده

این پژوهش در دو آزمایش متفاوت جهت بهینه‌سازی تکثیر درون‌شیشه‌ای آلسترومریا انجام شد. آزمایش اول: اثر بنزیل آمینوپورین (BAP) (صفر، 5/0، یک، 5/1و دو میلی‌گرم در لیتر) و ایندول بوتیریک اسید (IBA) (صفر، 1/0 و 2/0 میلی‌گرم در لیتر) روی جوانه‌های انتهایی (1-8/0 سانتی‌متر) رشد یافته از نوک شاخه‌های جوان رقم فوجی (Fuji) و آزمایش دوم: اثر 2,4-D (صفر، یک، دو، چهار، شش و هشت میلی‌گرم در لیتر)، پیکلورام (صفر، یک، دو، چهار، شش و هشت میلی‌گرم در لیتر) و BAP (صفر و 5/0 میلی‌گرم در لیتر) روی القاء کالوس جنینی از برگ‌های اولیه گیاهچه در گونه لیگتو بررسی شد. در هر دو آزمایش محیط‌کشت MS استفاده شد. در آزمایش اول: بالاترین تعداد شاخه‌ها در یک میلی‌گرم در لیتر BAP + 1/0 میلی‌گرم در لیتر IBA به‌دست آمد (27/4). نتایج نشان داد که افزایش غلظت BAP باعث کاهش طول شاخساره و میان‌گره شد که می‌تواند به سبب کاهش غالبیت انتهایی حاصل از اثر IBA باشد. بالاترین تعداد ریشه (25/3) در غلظت 2/0 میلی‌گرم در لیتر IBA بدون BAP و بالاترین تعداد ریزوم (5/3) در محیط‌کشت حاوی یک میلی‌گرم در لیتر BAP و بدون IBA به‌دست آمد. در آزمایش دوم: حداکثر کالوس جنینی متراکم (13/29 %) با دو میلی­گرم در لیتر پیکلورام و 5/0 میلی‌گرم در لیتر BAP، سپس توسط دو میلی‌گرم در لیتر 2,4-D و 5/0 میلی‌گرم در لیتر BAP به‌دست آمد. جهت پرآوری کالوس، هر دو نوع کالوس روی MS همراه با 4 میلی‌گرم در لیتر پیکلورام نگه‌داری شدند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Optimization of In Vitro Propagation of Alstroemeria by Apical Bud Culture, Callus Induction and Plant Regeneration

نویسندگان [English]

  • Fardin Nasri 1
  • Yavar Vafaee 2
  • Ali Akbar Mozafari 2
  • Seyed Najmedin Mortazavi 3
1 PhD Student, Department of Horticultural Sciences, Faculty of Agriculture, University of Gilan, Rasht
2 Assistant Professor, Department of Horticultural Sciences, Faculty of Agriculture, University of Kurdistan, Sanandaj
3 Associate Professor, Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan, Zanjan
چکیده [English]

This research was conducted at two different experiments to optimization of in vitro propagation Alstroemeria. Experiment 1: effect of BAP (0, 0.5, 1, 1.5 and 2 mg/l-1) and IBA (0, 0.1 and 0.2 mg/l-1) on apical buds (0.8-1 cm) grown from young’s shoots tips of Alstroemeria c.v. Fujie and experiment 2: effect of 2,4-D (0, 1, 2, 4, 6 and 8 mg/l-1), picloram (0, 1, 2, 4, 6 and 8 mg/l-1) and BAP (0 and 0.5 mg/l-1) were studied on embryonic callus induction from first seedling leaves in A. ligtu hybrid. The MS medium was used in both experiments. At experiment 1: the highest shoots number was obtained in 1 mg.l-1 BAP+0.1 mg.l-1 IBA (4.27). The results showed that increasing of BAP concentration caused decreasing of shoot and internode length due to apical dominant decreasing via IBA effect. The highest root number (3.25) was obtained in 0.2 mg.l-1 IBA concentration without BAP and the highest rhizome number was recorded at 1 mg/l-1 BAP without IBA (3.5). At experiment 2: maximum of compact embryonic callus (29.13%) was obtained with 2 mg/l-1 picloram and 0.5mg/l-1 BAP, followed by 2 mg/l-1 2,4-D and 0.5 mg/l-1 BAP. To callus proliferation, both types of callus were maintained on MS with 4 mg/l-1 picloram.

کلیدواژه‌ها [English]

  • Alstroemeria
  • Shoots tips
  • First seedling leaves
  • Organogenesis
  • Embryogenesis

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