بررسی تنوع ژنتیکی چهار نژاد از گوسفندان موجود در ایران با استفاده از نشانگرهای ریزماهواره‌ای

نوع مقاله: علمی - پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی‌ارشد گروه علوم دامی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان

2 استاد گروه علوم دامی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان

چکیده

با توجه به اهمیت شناسایی و حفظ تنوع ذخایر ژنتیکی کشور، چهار جمعیت از نژادهای گوسفند موجود در ایران (پاکستانی (25 رأس)، کرمانی (102 رأس)، ایران‌بلک (44 رأس) و لری‌بختیاری (25 رأس)) با استفاده از 4 نشانگر ریزماهواره‌ای (McMA2، HSC، OarHH35 و BM6444) از نظر تنوع ژنتیکی بررسی شدند. استخراج DNA ژنومی از تعداد 196 نمونه خون به روش نمکی بهینه ‌شده، انجام شد و واکنش‌های PCR با استفاده از چهار جفت آغازگر اجرا شد. نتایج نشان داد که تمامی جایگاه‌ها چندشکل هستند. تعادل هاردی- واینبرگ به روش آزمون مربع کای نشان داد که برخی ترکیبات مختلف جایگاه‌ها- جمعیت در حالت عدم تعادل قرار داشتند (05/0>P). بیش‌ترین تعداد آلل مشاهده ‌شده مربوط به جایگاه HSC در جمعیت پاکستانی (14 آلل) و کم‌ترین تعداد نیز از آن جایگاه HSC در جمعیت‌های کرمانی و ایران‌بلک (7 آلل) بود. حداکثر محتوای اطلاعات چندشکلی (PIC) در حالت ترکیب جمعیت- جایگاه برای جایگاه‌های McMA2، HSC، OarHH35 و BM6444 به‌ترتیب 9/0، 87/0، 87/0 و 86/0 بودند. در مجموع می‌توان نتیجه گرفت که جمعیت‌های گوسفند مورد بررسی در این تحقیق با توجه به جایگاه‌های مورد مطالعه، از تنوع ژنتیکی نسبتاً بالایی برخوردارند.

کلیدواژه‌ها


عنوان مقاله [English]

Genetic Diversity Analysis of Four Sheep Breeds Existing in Iran Using Microsatellite Markers

نویسندگان [English]

  • Mohammad Taghi Vajed Ebrahimi 1
  • Mohammad Reza Mohammadabadi 2
  • Ali Esmailizadeh 2
1 MSc Graduated, Department of Animal Sciences, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman
2 Professor, Department of Animal Sciences, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman
چکیده [English]

Considering the importance of identification and maintaining of genetic diversity in native animals, four sheep breeds existing in Iran (Pakistani (25), Kermani (102), Iran Black (44) and Lory-Bakhtiyari (25)) were evaluated in terms of genetic diversity, using four microsatellite markers McMA2, HSC, OarHH35 and BM6444. Genomic DNA was extracted from 196 blood samples by optimized salting-out DNA extraction procedure and PCR was performed using four primer pairs. The results showed that all loci are polymorph. Hardy-Weinberg equilibrium using chi-square test showed that some of the various components of loci - population were in Hardy-Weinberg disequilibrium (P<0.05). The highest number of alleles were observed for HSC of Pakistani population (14 alleles) and the lowest number of alleles were in HSC in Kermani and IranBlack populations (7 alleles). The polymorphic information content (PIC) of McMA2, HSC, OarHH35 and BM6444 were 0.9, 0.87, 0.87 and 0.86 respectively. In general, it can be concluded that all studied sheep populations have wide genetic diversity based on studied loci.

کلیدواژه‌ها [English]

  • Heterozygosity
  • Population
  • Alleles
  • Polymorphism

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