ارزیابی تنوع ژنتیکی زیره با استفاده از نشانگرهای ریزماهواره چوچاخ (Eryngium planum)

نوع مقاله: علمی - پژوهشی

نویسندگان

1 دانشجوی کارشناسی‌ارشد، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل، زابل

2 استادیار گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل، زابل

3 مربی پژوهشی، گروه زراعت و اصلاح نباتات، پژوهشکده کشاورزی، دانشگاه زابل، زابل

چکیده

گیاهان دارویی اهمیت خاصی در تأمین بهداشت و سلامتی جوامع از لحاظ درمان و پیشگیری از بیماری‌ها دارند در این میان گیاه دارویی زیره (سیاه و سبز) در جایگاه خاصی قرار گرفته است. تعیین فاصله ژنتیکی بین دو گروه (10 جمعیت) زیره سبز و سیاه براساس نشانگرهای SSR، هدف این تحقیق می‌باشد. در زیره سبز تعداد قطعه از 2 (Ealp024) تا 5 (Ealp245 و Ealp741 ) متغیر با میانگین 25/3 و در زیره سیاه از 2 (Ealp017، Ealp741، Ealp1349 و EalpD268) تا 6 (Ealp1479 و Ealp024) با میانگین 5/3 به‌دست آمد. میزان شاخص چندشکلی در زیره سبز از 48/0 (Ealp024) تا 89/0 (Ealp017) با میانگین 61/0 و در زیره سیاه از 32/0 (Ealp741) تا 8/0 (Ealp1479) با میانگین 61/0 مشاهده گردید. بیش‌ترین میزان تنوع شانن (28/0) و تنوع نی (20/0) مربوط به زیره سیاه نیشابور و زیره سیاه جندق و کم‌ترین میزان شاخص تنوع شانن (057/0 و 056/0) و نی (04/0) مربوط به زیره سبز سبزوار و زیره سبز نیشابور بود. دورترین جمعیت‌ها در بین زیره‌های سبز، زیره سبز سبزوار و زیره سبز گز برخوار و در بین زیره‌های سیاه، زیره سیاه کرمان و زیره سیاه نیشابور بودند. نتایج حاصل از تجزیه واریانس مولکولی نشان داد که 85 درصد تغییرات ژنتیکی، درون جمعیت‌ها و 15 درصد در بین جمعیت‌ها بود. نشانگرهای SSR که در این تحقیق مورد استفاده قرار گرفت برگرفته از چوچاخ بودند و توانستند در زیره سبز و سیاه نیز تکثیر شوند که می‌تواند دلیلی بر روند تکاملی و مناطق حفاظت شده مشترک بین زیره و چوچاخ باشد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Genetic Evaluation of Cumin and Caraway Using Eryngium planum Microsatellite Markers

نویسندگان [English]

  • Zahra Ahmand 1
  • Leila Fahmideh 2
  • Bahman Fazeli-Nasab 3
1 MSc Student, Department of Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol
2 Assistant Professor, Department of Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol
3 Lecturer Research, Department of Agronomy and Plant Breeding, Agricultural Research Institute, University of Zabol, Zabol
چکیده [English]

Medicinal plants have special importance in terms of public health promotion and disease prevention with treatment. Among these medicinal plants, black and green cumin have a special place. The aim of this study was to evaluate the genetic similarity and genetic distance between two group (10 population) of cumin and caraway based on SSR markers. In Cumin, the number of allele amplified per primer was different from 2 (Ealp024) to 5 (Ealp245 and Ealp741) with average 3.25 and also in caraway, was from 2 (Ealp017, Ealp741, Ealp1349 and EalpD268) from 6 (Ealp1479 and Ealp024) with average (3.5). In Cumin, diversity index was different from 0.48 (Ealp024) to 0.89 (Ealp017) with average 0.61 and also in caraway was from 0.32 (Ealp741) to 0.8 (Ealp1479) with average (0.61). The highest Shanon diversity (0.28) and Nei diversity (0.20) were belong to caraway population of Neishabor and Jandagh and the lowest Shanon diversity (0.057 and 0.056) and Nei diversity (0.04) were belong to cumin population of Sabzvar and Neishabor. The fartherest population among cumin were cumin of Sabzvar and Gazbarkhoar and among caraway were caraway of Kerman and Neishabor. Partitioning variations within and between populations, using an analysis of molecular variance (AMOVA), showed that 13% and 87% of the total genetic variation respectively existed between and within growing regions. Eryngium planum Microsatellite Markers that were used and amplified in Cumin and Caraway approved evolution process and the present conserve sequence between Cumin, Caraway and Eryngium planum.

کلیدواژه‌ها [English]

  • Genetic diversity
  • Carum carvi
  • Cuminum cyminum
  • SSR

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