کاربرد الکتروفورز دوبعدی در شناسایی منابع مقاومت و حساسیت بیماری زنگ زرد (Puccinia striiformis f. sp. tritici West.) در گندم نان (.Triticum aestivum L)

نوع مقاله : علمی - پژوهشی

نویسنده

استادیار اصلاح‌نباتات بخش کشاورزی، دانشگاه پیام‌نور، تهران، ایران

چکیده

بیماری زنگ زرد گندم ازجمله تنش‌های زیستی است که همه‌ساله کشاورزان زیادی را متحمل خسارت می‌نماید. مطالعه حاضر برای بررسی پاسخ پروتئینی گیاه گندم در حضور و عدم حضور بیماری زنگ زرد با کمک روش الکتروفورز دوبعدی صورت پذیرفت. شاخص‌های مقاومت نسبی شامل تیپ آلودگی، شدت بیماری، ضریب آلودگی، سطح زیر منحنی توسعه بیماری و نرخ آلودگی ظاهری ازجمله شاخص‌هایی هستند که برای شناسایی مقاومت یا حساسیت ارقام مختلف مورد استفاده قرار می‌گیرند در این مطالعه، ابتدا شاخص‌های مذکور برای 14 رقم گندم نان طی سه قرائت 7 روزه به‌دست آمدند. ارقام با استفاده از تجزیه خوشه‌ای مبتنی بر شاخص‌های مقاومت، به سه گروه حساس، مقاوم و نیمه‌مقاوم تقسیم‌، دو رقم پیشگام و شهریار به‌ترتیب به‌عنوان مقاوم‌ترین و حساس‌ترین ارقام شناخته شدند. تغییرات در الگوی بیان پروتئین‌ها با استفاده از روش الکتروفورز دوبعدی در دو رقم مذکور موردبررسی قرار گرفت. نتایج حاصل از تجزیه آماری نشان داد، که از مجموع 442 لکه پروتئینی تکرارپذیر، 27 لکه پروتئینی، تغییر بیان نشان دادند. از کل پروتئین‌های دارای تغییر بیان معنی‌دار، 17 لکه دچار افزایش بیان و 10 لکه دچار کاهش بیان شدند. به‌طور‌کلی، نتایج به‌دست آمده از این مطالعه نشان داد که تفاوت در الگوی پروتئوم دو رقم مقاوم و حساس گندم می‌تواند تغییر در میزان بیان آنزیم‌ها و پروتئین‌های درگیر در مقاومت را نشان دهد.

کلیدواژه‌ها

عنوان مقاله [English]

Application of Two-dimensional Electrophoresis to Identify the Resistance and Sensitivity Sources of Yellow Rust Disease (Puccinia striiformis f. sp. tritici West.) in Bread Wheat )Triticum aestivum L.(

نویسنده [English]

  • Mehdi Kakaei
Assistant Professor, Department of Agriculture (Plant Breeding), Payame Noor University, Tehran, Iran
چکیده [English]

Yellow rust is one of the major diseases of wheat that suffers a loss many farmers. The purpose of the current study, was determining the wheat proteome response to presence and non-presence of yellow rust disease using Two-dimensional gel electrophoresis. Relative resistance indices including infection type, severity of disease, contamination coefficient, surface under the disease development curve and apparent contamination rates are indicators that are used to identify the resistance or sensitivity of different cultivars. In this study, firstly these indicators studied for 14 bread wheat cultivars was obtained during three 7-day readings. The studied wheat cultivars were classified into three susceptible, resistant and semi-resistant groups using cluster analysis based on resistance indices. Accordingly, two Pishgam and Shahriar cultivars were identified as the most resistant and sensitive cultivars, respectively. Changes in the pattern of expression of proteins in two resistant (Pishgam) and susceptible (Shahriyar) cultivars by tow dimensional electrophoresis of were investigated. The results of statistical analysis showed that 27 protein spots out of 442 repeated protein spots showed a change in expression. Significant changes in total proteins, 17 spots, increased expression, and 10 spots decreased expression. In general, the results of this study showed that the difference in proteome pattern of two resistant and susceptible wheat cultivars to yellow rust could indicate a change in the expression of the enzymes and proteins involved in resistance, and possibility to resist pathogenic agents.

کلیدواژه‌ها [English]

  • Solution protein
  • Disease
  • Susceptible
  • Relative resistance

مراجع

Ali, S., Shah, S. J. A. and Ibrahim, M. 2007. Assessment of wheat breeding lines for slow yellow rusting (Puccinia striiformis West. tritici). Pakistan Journal of Biological Science, 10: 3440-3444.
Amini, F. and Ehsanpour, A. 2009. Investigation of Protein Changes in Lycopersicon esculentum cv. Isfahani under salinity stress using two-dimensional electrophoresis technique. Iranian Journal of Plant Biology, 1 (1): 13-24 (In Persian)
Berenbaum, M. R. 1995. Chemical defence: theory and practice. Proc Natl Acad Sci USA 92: 2-8.
Bhadauria, V., Popescu, L., Zhao, W. S. and Peng, Y. L. 2007. Fungal transcriptomics. Microbiological Research, 1: 285-298.
Bowers, W.S. 1980. Chemistry of plant /insect interaction. In Insect Biology in the future, edited by M. Locke and D. S. Smith, New York: Yadavaran Publishing, 633pp.
Broers, L. H. M., Subias, X. C. and Atilano, R. M. L. 1996. Field assessment of quantitative resistance to yellow rust in ten spring bread wheat cultivars. Euphytica, 90: 9-16.
Damerval, C., Vienne, D., Zivy, M. and Thiellement, H. 1986. Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat-seedling proteins. Electrophoresis, 7: 52-54.
Ekhvat, S. M. 2003. Plant Disease (Theoric). PNU Publications. 128 pp.
Gade-kahriz, S. J. and Parmoon, B. C. 2015. Proteomics in Plant Sciences. (Compilation) Haghshenas Publications. 409-416 pp.
Green, T. R. and Ryan, C. A. 1972. Wound-induced proteinase inhibitor in plant leaves: a possible defense mechanism against insects. Science, 175: 776-777.
Gygi, S. P., Rist, B. and Aebersold, R. 2000. Measuring gene expression by quantitative proteome analysis. Current Opinion Biotechnology, 11 (4): 396-401.
Kafie, M. and Mahdavi-Damghani, A. S. 2001. Mechanism of plant resistance to environmental stress. University Ferdowsi Mashhad Publishshing, 467pp.
Khanjani, M. 2009. Field crop Pests in Iran (Insect & Mites). Bu-Ali Sina university press. 467pp.
Kakaei, M., Mazahery-laghab, H. and Mostafaie, A. 2017. Study of protein changes in alfalfa (Medicago sativa L.) in normal conditions and under the tension from alfalfa weevil (Hypera postica Gyll.) feeding. Journal of Agricultural Biotechnology, 9 (1): 49-66.
Milus, E. A. and Line, R. F. 1986.Gene action for inheritance of durable, hightemperature adult plant resistance to stripe rust to wheat. Phytopathology, 76: 435-441.
Parlevliet, J. E. 1988. Resistance of the non-race-specific type. pp. 501-525 In: Roelfs, A. P. and Bushnell, W. R. The Cereal Rusts, Vol II: Disease, Distribution, Epidemiology and Control. Academic Press, Orelando, USA.
Pathan, A. K. and Park, R. F. 2006. Evaluation of seedling and adult plant resistance to leaf rust in European wheat cultivars. Euphytica, 149: 327-342.
Peterson, R. F., Campbell, A. B. and Hannah, A. E. 1948. A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Canadian Journal of Research, 26: 496-500.
Roelfs, A. P. and Bushnell, W. R. 1985. The Cereal Rusts. Vol II: Disease, Distribution, Epidemiology and Control. Academic Press, Orlando, USA. 606 pp.
Seyyedi-Sahebari, F. 2012. Evaluation of reaction of alfalfa ecotypes to alfalfa weewil, Hypera postica Gyll. (Coloptera: Curculionidae) Iranian Plant Protection Research Institute, 60 pp.
Shah, S. J. A., Khan, A. J., Azam, F., Mirza, J. I. and Rehman, A. U. 2003. Stability of rust resistance and yield potential of some ICARDA bread wheat lines in Pakistan. Pakistan Journal of Scientific and Industrial Research, 46: 443-446.
Smith, C. M. 1989. Plant Resistance to Insects, A fundamental approach. John Wiley and Sons. New York. 286 p.
Stubbs, R. W., Prescot, J. M., Saari, E. E. and Dubi, H. J. 1986. Cereal Disease Methodology Manual. Centro International de Mejoramiento de Maize y Trigo (CIMMYT), Mexico, DF.
Van Loon, L. C., Rep, M. and Pieterse, C. M. J. 2006. Significance of Inducible Defense-related Proteins in Infected Plants. Phytopathology, 44: 135-162.
Valizadeh Kamran, R., Toorchi, M., Moghadam, M. and Mohammadi, H. 2015. The Proteomic Analysis of Spring Barley Leaves under Short term Cold Stress. Genetic Engineering and Biosafety Journal, 4(1): 67-78.
Vanderplank, J. E. 1963. Plant Diseases. Epidemics and Control: Academic Press, New York, USA. 349 pp.
Wilcoxson, R. D., Skovmand, B. and Atif, A. H. 1975. Slow rusting of wheat varieties in the field correlated with stem rust severity on detached leaves in the greenhouse. Plant Disease Reporter, 58: 1085-1087.
Zahravi, M., Ibrahimnejad, Sh. and Afshari, F. 2011. Evaluation of relative resistance fields and relationships between components of yellow rust resistance to bread wheat germplasm. Journal of Seed and Plant Seedlings, 4: 663-684.
Zivy, M. and de Vienne, D. 2000. Proteomics: A link between genomics, genetics and physiology. Plant Molecular Biology, 44: 575-580.
دوفصلنامه فن آوری زیستی در کشاورزی(علمی-پژوهشی)
دوره 9، شماره 1
خرداد 1397
صفحه 11-22

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