بررسی همراهی گونه‌های ویروسی مختلف با بیماری موزائیک انجیر در ایران

نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه بیماری‌شناسی گیاهی، دانشکده کشاورزی و منابع طبیعی، واﺣﺪ ﻋﻠﻮم و ﺗﺤﻘﻴﻘﺎت دانشگاه آزاد اسلامی، تهران، ایران

2 استادیار، گروه بیماری‌شناسی گیاهی، دانشکده کشاورزی و منابع طبیعی، واﺣﺪ ﻋﻠﻮم و ﺗﺤﻘﻴﻘﺎت دانشگاه آزاد اسلامی، تهران، ایران

3 استاد پژوهش، بخش تحقیقات بیوتکنولوژی میکروبی، پژوهشکده بیوتکنولوژی کشاورزی ایران (ABRII)، سازمان تحقیقات، آموزش و ترویج کشاورزی (AREEO)، کرج، ایران

4 ﺩﺍﻧﺸﻴﺎﺭ، ﮔﺮﻭﻩ ﺑﻴﻤﺎﺭﻱ‌ﺷﻨﺎﺳﻲ ﮔﻴﺎﻫﻲ، ﺩﺍﻧﺸﻜﺪﻩ ﻛﺸﺎﻭﺭﺯﻱ، ﺩﺍﻧﺸﮕﺎﻩ ﺗﺮﺑﻴﺖ ﻣﺪﺭﺱ، تهران

چکیده

بیماری موزائیک انجیر مهم‌ترین بیماری خسارت زا در انجیر است و چندین ویروس از خانواده‌های مختلف با ژنوم از نوع آر.ان.ای (RNA) و دی.ان.ای (DNA) در آن نقش دارند. در این تحقیق حضور مهم‌ترین ویروس­های با ژنوم آر.ان.ای دخیل در بیماری شامل Fig mosaic virus (FMV)، Fig latent virus-1 (FLV-1)،Fig cryptic virus (FCV) ، Fig fleck-associated virus (FFKaV)، Fig leaf mottle associated virus-1 (FLMaV-1)، Fig leaf mottle associated virus-2 (FLMaV-2)، Fig leaf mottle associated virus-3 (FLMaV-3) و Fig mild mottle associated virus (FMMaV) در انجیرکاری‌های مختلف کشور موردبررسی قرار گرفت. نمونه‌برداری‌ها از 14 استان انجام شد و شامل 611 نمونه برگی دارای علائم موزائیکی بود. پس از استخراج آر.ان.ای دو رشته‌ای با ستون سلولزی، سی.دی.ان.ای (cDNA) با استفاده از آغازگر تصادفی ساخته شد. واکنش زنجیره‌ای پلی‌مراز برای هرکدام از این ویروس‌ها با آغازگر اختصاصی مربوط به هر ویروس انجام گرفت. درنهایت بعد از مشخص شدن مناطق آلوده، آلودگی‌های مخلوط بین خانواده‌های مختلف ویروسی موردبررسی و FMV مشخص شد. نتایج حاکی از وجود آلودگی در تمام استان‌های موردمطالعه بود و هم‌چنین بیش از نیمی از نمونه‌های جمع‌آوری شده حداقل به یکی از ویروس‌ها آلوده بودند. بعضی از ویروس‌ها مانند FMV نیز در بعضی از مناطق مثل استان فارس ردیابی نشد. بیش‌ترین میزان آلودگی مربوط به FMV و FFKaV با 7/34 و 2/14 درصد و کم‌ترین مربوط به FMMaV با 4/4 درصد آلودگی بود. بیش‌ترین میزان آلودگی مخلوط مربوط به حضور FMV با اعضاء خانواده Closteroviridae بود.

کلیدواژه‌ها

عنوان مقاله [English]

Study on the Association of Different Virus Species with Fig Mosaic Disease in Iran

نویسندگان [English]

  • Athar Alishiri 1
  • Farshad Rakhshandehroo 2
  • Gholamreza Salehi Jouzani 3
  • Masoud Shams Bakhsh 4
1 PhD Student, Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Assistant Professor, Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 Professor, Department of Microbial Biotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj , Iran
4 Associate Professor, Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
چکیده [English]

Fig mosaic disease which is known as one of the most important impacting viral diseases of fig, is caused by several DNA and RNA viruses belonging to the different genera and families. The objective of the present study was to evaluate the presence of the most important RNA viral agents causing the disease including: Fig mosaic virus (FMV), Fig latent virus-1 (FLV-1), Fig cryptic virus (FCV), Fig fleck-associated virus (FFKaV), Fig leaf mottle associated virus-1 (FLMaV-1), Fig leaf mottle associated virus-2 (FLMaV-2), Fig leaf mottle associated virus-3 (FLMaV-3) and Fig mild mottle associated virus (FMMaV) in different fig cultivations of the country. Sample collection was done from 14 provinces and 611 symptomatic fig leaves. The cDNA of the samples was synthesized using the isolated dsRNAs with cellulose column and random primer. The molecular identification was performed using PCR and specific primers for each virus. After characterization of the infected regions, the results of identification of mixed infections by different viruses showed that the FMV. The results indicated that all of the studied provinces and more than half of the collected samples are infected with at least one of the viruses. Some of the viruses such as FMV were not detected in certain visited regions such as Fars province. The FMV and FFKaV with infection rates of 34.7% and 14.2% and FMMaV with 4.4% showed the highest and lowest infection rates, respectively. The highest level of the mixed infection was observed for FMV and the members of the Closteroviridae family.

کلیدواژه‌ها [English]

  • Fig mosaic disease
  • Fig mosaic virus
  • Mixed infection
  • RNA viruses

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دوفصلنامه فن آوری زیستی در کشاورزی(علمی-پژوهشی)
دوره 9، شماره 1
خرداد 1397
صفحه 55-67

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