شناسایی نشانگر(های) RAPD پیوسته به ژن(های) کنترل کننده زمان گلدهی در جمعیت 1F بادام حاصل از تلاقی کنترل شده (♂) تونو × (♀) شاهرود 12

نوع مقاله: علمی - پژوهشی

نویسندگان

1 استادیار گروه علوم باغبانی و فضای سبز دانشکده کشاورزی دانشگاه ملایر، ملایر

2 دانشیار گروه علوم باغبانی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 استاد گروه علوم باغبانی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

4 دانشیار بخش باغبانی،موسسه اصلاح، تهیه نهال و بذر کرج

چکیده

در این تحقیق زمان گلدهی و برخی صفات مورفولوژیکی به­مدت دو سال در جمعیت 1F شامل 72 نتاج حاصل از تلاقی رقم­های ’تونو ‘(میان گل) و ’شاهرود 12 ‘دیرگل مورد ارزیابی قرار گرفت. در این تحقیق برای شناسایی نشانگرهای همبسته با ژن زمان گلدهی، از روش تجزیه تفرق توده­ای با استفاده از 150 آغازگر RAPD در توده انتخاب شده و نهایتاً کل جمعیت مورد بررسی، استفاده شد. نتایج تأیید کرد که توارث زمان گلدهی در نتاج ارزیابی شده به­صورت کمی می­باشد. زمان گلدهی در نتایج محدوده وسیعی را نسبت به والدین نشان دادند، هرچند که برخی از نتاج زودتر از والد میان گل ’تونو‘ به گل رفتند. نتایج نشان داد که نشانگرهای BA-17600,1000،BC-05320 ،BC-06800 ، BC-141750، BC-17600،BC-20250 ، OPC-05850 و OPC-09700,1100 با دیرگلدهی و BA-04720، BB-10630، BC-092000، BD-12510 و OPC-12350 با زودگلدهی در ارتباط بودند. پس از تهیه نقشه ژنتیکی جمعیت مورد بررسی، تجزیه QTL برای زمان گلدهی انجام شد. نتایج نشان داد که آغازگر BA-17به­میزان 4 سانتی­مورگان با یکی از مکان­های ژنی کنترل­کننده دیرگلدهی فاصله دارد. همچنین آغازگرهای OPC-09 و BA-04 به­ترتیب در فاصله 2 و 3 سانتی­مورگان از یکی از ژن­های کنترل­کننده دیرگلدهی و زودگلدهی قرار گرفتند. نشانگرهای ژنتیکی پیوسته با زمان گلدهی در بادام بسیار مهم می­باشد چرا که استفاده از این نشانگرها در جهت انتخاب مستقیم برای شناسایی واریته­های مناسب از نظر زمان گلدهی از بین ژنوتیپ­های زودگل موجب صرفه­جویی در زمان و هزینه می­گردد. 

کلیدواژه‌ها


عنوان مقاله [English]

Identification of RAPD Marker(s) Linked to the Gene (s) Controlling Flowering Time in F1 (♀) Almond Population from Controlled Crosses of ‘Tuono’ (♂) × ‘Shahrood-12’

نویسندگان [English]

  • mousa rasouli 1
  • mohammad reza fatahi moghadam 2
  • zabih allah zamani 3
  • ali imani 4
  • ali ebadi 3
چکیده [English]

In this study flowering time and some morphological traits were evaluated during two years in a F1 almond progeny of seventy two seedlings from the cross between ‘Tuono’ )intermediate flowering(and ‘Shahrood-12’)late flowering( cultivars. Modified-bulk segregant analysis with the application of the 155 RAPD primers, spanning the whole almond genome were used to identify molecular markers linked to flowering time in several selected descendants from the studied almond progeny. Results showed a quantitative inheritance of this trait in the progeny and then to all 72 progenies. The seedlings evaluated showed a wide range of flowering time between both progenitors and some of these descendants were earlier than the intermediate flowering progenitor ‘Tuono’. The results showed that BA-17600,1000, BC-05320, BC-06800, BC-141750, BC-17600, BC-20250, OPC-05850 and OPC-09700 markers were linked to late blooming and BA-04720,BB-10630,BC-092000,BD-12510andOPC-12350 were linked to early blooming time. After construction of the genetic map of population, QTL analysis was performed for flowering time. The results showed that BA-17 primer had 4 cM distance from one of the late flowering time loci. Also,the OPC-09 and BA-04 primers were located at 2 and 3 cM distances from one of the genes controlling early and late flowering time, respectively.Identification of genetic markers linked to blooming time in almond are very important, because utilization of these markers will help the indirect selection of genotypes for desirable bloom time in early generation to saving time and effort. According to the obtained results, with the development of these markers, the strategies of marker assisted selection can be used in breeding programs of almond, apricot, peach and other Prunus species. 

کلیدواژه‌ها [English]

  • Prunus dulcis
  • Flowering time
  • Genetic mapping
  • QTL
  • BSA
  • Marker-assisted selection
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