وقوع ویروس موزائیک گوجه‌فرنگی آلوده‌کننده کدوئیان در برخی از استان‌های ایران

نوع مقاله: علمی - پژوهشی

نویسندگان

1 فارغ التحصیل کارشناسی‌ارشد گروه بیماری‌شناسی گیاهی، دانشکده کشاورزی و منابع طبیعی، واحد علوم و تحقیقات دانشگاه آزاد اسلامی، تهران

2 استادیار گروه بیماری‌شناسی گیاهی، دانشکده کشاورزی و منابع طبیعی، واحد علوم و تحقیقات دانشگاه آزاد اسلامی، تهران

چکیده

چکیده
    ویروس موزائیک گوجه‌فرنگی Tomato mosaic virus (ToMV) یکی از مخرب‌ترین ویروس‌های آلوده‌کننده سبزیجات می‌باشد. این تحقیق به‌منظور برآورد وضعیت پراکنش ToMV در برخی از کدوئیان شامل خیار، کدو و هندوانه صورت گرفت. برای این منظور طی سال‌های 1387 تا 1389 از چندین مزرعه از استان‌های مختلف کشور نمونه‌برداری انجام شد. نمونه‌های برگی از بوته‌های علائم‌دار انتخاب و با استفاده از روش الیزای مستقیم مورد ارزیابی قرار گرفتند. نمونه‌هایی که آلوده تشخیص داده شدند برای ادامه کار انتخاب شدند. تعیین دامنه میزبانی با استفاده از مایه‌زنی مکانیکی گیاهان محک انجام شد. تأیید آلودگی نمونه‌ها با استفاده از آزمون مولکولی RT-PCR و با Total RNA استخراج شده از نمونه‌ها انجام شد. این آزمون به روش semi-nested RT-PCR و با استفاده از آغازگر عمومی توباموویروس‌ها و آغازگر اختصاصی مربوط به گونه ToMV انجام پذیرفت. باند مورد انتظار معادل 675 جفت باز برای نمونه‌های آلوده به‌دست آمد. نتایج آزمون‌های الیزا، زیست‌سنجی و RT-PCR وجود ویروس موزائیک گوجه‌فرنگی را در کدوئیان مختلف شامل خیار و کدو مسمای از مراغه، هندوانه از ارومیه، خیار از زاهدان و رودان و کدو مسمائی از ورامین اثبات نمود. نتایج این تحقیق نشان داد کدوئیان کاشته شده در ایران به ویروس موزائیک گوجه‌فرنگی آلوده می‌باشند و لازم است که در اتخاذ برنامه‌های مدیریت کنترل بیماری‌های کدوئیان اجرای اصول مبارزه با ToMV نیز مدنظر قرار گیرد.
 

کلیدواژه‌ها


عنوان مقاله [English]

Tomato Mosaic Virus Occurrence Infecting Cucurbits In Some Provinces of Iran

نویسندگان [English]

  • Athar Alishiri 1
  • Farshad Rakhshandehroo 2
1 M. A. Department of Plant Pathology, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran
2 Assistant Professor, Department of Plant Pathology, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran
چکیده [English]

Abstract
Tomato mosaic virus is one of the most damaging viruses infecting vegetable crops. This study was conducted to estimate the distribution of ToMV in some cucurbit plants including cucumber, squash and watermelon. Toward this aim, during the years 2008 to 2010 samples were taken from numbers of cucurbit cultivations of different provinces of Iran. Leaf samples were selected from symptomatic plants and tested with DAS-ELISA method. Samples with positive reaction with antisera in ELISA were further selected for subsequent assays. Bioassay was done using with the herbaceous host plants and mechanical inoculations. RT-PCR was done with the total RNA extracted from the leaf samples. Samples were amplified by semi-nested RT-PCR using with the universal tobamovirus primer and Tomato mosaic virus (ToMV) specific primer pairs. A desired DNA fragment with a size about 675 bp was amplified for ToMV infected plants. Results of the ELISA, RT-PCR tests and bioassay confirmed the presence of ToMV in different cucurbit crops as follow: cucumber and squash in Maragheh district, watermelon in Orumia district, cucumber in Zahedan and Roodan districts and squash in Varamin district. Results of this study indicated that cultivated cucrbits in Iran are infected with ToMV. It is necessary to consider the ToMV control management strategy in general control management scheme for cucurbit plants.

کلیدواژه‌ها [English]

  • Cucurbits
  • Tomato mosaic virus
  • Incidence
  • Semi-nested RT-PCR
Aghamohammadi, V., Rakhshandehroo, F. and Shams-bakhsh, M. 2011. First report of Tomato mosaic virus in eggplant in Iran. Journal of Plant Pathology, 93: S4.63-S4.89.

Aghamohammadi, V., Rakhshandehroo, F., Shams-bakhsh, M. and Palukaitis, P. 2013. Distribution and genetic diversity of Tomato mosaic virus isolates in Iran. Journal of plant pathology, 95(2): 339-347.

Ale-Yassin, K. and Izadpanah, K. 1995. Electron microscopical and serological identification of Beet curly top virus (BCTV) and its relation to symptoms observed in various plants in Fars. Proceeding of 12th Iranian plant protection. Congress., Karaj. Ministry of Agriculture, Tehran, Iran, 124 Pp.

Alishiri, A., Rakhshandehroo, F. and Zamanizadeh, H. 2011. First report of Tobacco mild green mosaic virus infecting tomato in Iran. New disease reports, 23: 30.

Alishiri, A., Rakhshandehroo, F. and Zamanizadeh, H. 2010. Distribution and genetic variation of Tobacco mosaic virus in cucurbit fields of east Azerbaijan. Proceeding of 11th Iranian Genetic Congress., University of shahid beheshti , Tehran, Iran. p: 100681.

Alishiri, A., Rakhshandehroo, F. and Zamanizadeh, H. 2012. Occurrence of Pepper mild mottle virus (PMMV) and Cucumber green mottle mosaic virus (CGMMV) from some vegetable cultivation region of Iran. Proceeding of 20th Iranian plant protection. Congress., Shiraz. Ministry of Agriculture, Fars, Iran, pp: 806.

Bananej, K., Kheyr-Pour, A. and Ahoonmanesh, A. 1998. Identification of Watermelon chlorotic stunt virus WmCSV in Iran. Proceeding of 13th Iranian plant protection. Congress., Karaj. Ministry of Agriculture, Tehran, Iran, pp: 194.

Bananej, K. and Vahdat, A. 2008. Identification, distribution and incidence of viruses in field-grown cucurbit crops of Iran. Journal of phytopathology mediteranea, 47: 247-257.

Bananej, K., Desbiez, C., Wipf-Scheibel, C., Vahdat, I., Kheyr-Pour, A., Ahoonmanesh, A. and Lecoq, H. 2006. First report of Cucurbit aphid-borne yellows virus in Iran causing yellows on four cucurbit crops. Plant diseases, 90(4): 526.

Channuntapipat, C., Sedgley, M. and Collins, G. 2001. Sequences of the cDNAs and genomic DNAs encoding the S1, S7, S8 and Sf alleles from almond. Prunus duleis. Theoretical and applied genetics, 103: 1115-1122.

Clark, M. and Adams, A. N. 1977. Characterization of the microplate methods of enzyme link immunosorbent assay (elisa) for the detection of plant viruses. Journal of genetic virology, 34: 475-483.

Ebrahim-Nesbat, F. 1972. A report on isolation of Watermelon mosaic virus in Iran. Iranian Journal of Plant Pathology, 8(1): 10.

Fukuda, M., Meshi, T., Okada, Y., Otsuki, Y. and Takebe, I. 1981. Correlation between particle multiplicity and location on virion RNA of the assembly initiation sites for viruses of the Tobacco mosaic virus group. Procceding of National Academy of Sciences USA, 78: 4231-4235.

Ghorbani, S. 1986. Isolation of Cucumber green mottle mosaic virus (CGMMV) in Markazi province. Proceeding of 8th Iranian plant protection. Congress., Isfahan. Faculty of Agriculture, Isfahan, Iran pp: 121.

Ghorbani, S. 1988. Isolation of ZYMV in the Tehran province. Iranian Journal of plant pathology, 24(1-4): 13-15.

Ishibashi, K., Mawatari, N., Miyashita, S., Kishino, H., Meshi, T. and Ishikawa, M. 2012. Coevolution and hierarchical interactions of Tomato mosaic virus and the resistance gene Tm-1. PLoS Pathogens, 8: 1-11.

Izadpanah, K. 1987. Squash mosaic virus as the cause of melon veinbanding in Iran. Journal of Phytopathology, 120 (3): 276-282.

Keshavarz, T. and Izadpanah, K. 2004. Report of Cucurbit yellow stunting disorder virus (Genus crinivirus) in Iran. Proceeding of 16th Iranian plant protection. Congress., Tabriz. Faculty of Agriculture, Tabriz, Iran pp: 264

Kim, O. K., Lee, K. W. and Natsuaki, K. T. 2009. Occurrence and molecular characterization of Kyuri green mottle mosaic virus isolated from oriental melon in Korea. Journal of Agricultural Science, Tokyo university agriculture, 54: 71-78.

Letschert, B., Adam, G., Lesemann, D. E., Willingmann, P. and Heinze, P. 2002. Detection and differentiation of serologically cross-reacting tobamoviruses of economical importance by RT-PCR and RT-PCR-RFLP. Journal of virological methods, 106: 1-10.

Lisa, V., Milne, R. G., Accotto, G. P., Boccardo, G., Caciagli, P. and Parvizy, R. 1988. Ourmia melon virus, a virus from Iran with novel properties. Annals of Applied Biology, 112: 291-302.

Manochehri, A. 1968. Plant virus diseases. Tehran University Press, Tehran, Iran, 1189: 194.

Massumi, H., Samei, A., Hosseini Pour, A., Shaabanian, M. and Rahimian, H. 2007. Occurrence, distribution and relative incidence of seven viruses infecting greenhouse-grown cucurbits in Iran. Plant diseases, 91(2): 159-163.

Massumi, H., Shaabanian, M., Hosseini Pour, A., Heydarnejad, J. and Rahimian, H. 2009. Incidence of viruses infecting tomato and their natural hosts in the southeast and central regions of Iran. Plant diseases, 93: 67-72.

Massumi, H., Shaabanian, M., Heydarnejad, J., Hosseini Pour, A. and Rahimian, H. 2011. Host range and phylogenetic analysis of Iranian isolates of Zucchini yellow mosaic virus. Journal of Plant Pathologgy, 93(1): 187-193.

Safaeezadeh, M. 2007. First report of Melon necrotic spot virus and Zucchini yellow fleck virus in cucurbits in Iran. Journal of Plant Pathology, 89(2): 292.

Soler, S., Prohens, J., Lopez, C., Aramburu, J., Galipienso, L. and Nuez, F. 2010. Viruses infecting tomato in Valencia, Spain: occurrence, distribution and effect of seed origin. Journal of phytopathology, 158: 797-805.

Tomita, T., Sekine, K. T., Mizumoto, H., Sakamoto, M., Murai, J., Kiba., A., Hikichi, Y., Suzuki, K. and Kobajashi, K. 2011. Genetic basis for the hierarchical interaction between tobamo virus spp. and L resistance gene alleles from different pepper species. Molecular Plant-Microbe Interaction, 24: 108-117.

Van Regenmortel, M. H. V. 1981. Handbook of plant virus infections and comparative diagnosis, p. 541; (ed), Kurstak, E. Elsevier/North Holland Biomedical Press, Amesterdam.

Weideman, H. L. and Mostafawy, M. 1972. Watermelon mosaic virus-2 in Iran and its transmission by different aphid species. Iranian journal of plant pathology, 8(2): 46-51.