بررسی پروفـیل باندهای ساپونـین‌های برگ 3 رقم یونـجه (Medicago sativa L.) با مقاومت‌های متفاوت نسبت به سرخرطومی برگ یونجه (Hypera postica Gyll.) با به‌کارگیری TLC

نوع مقاله: علمی - پژوهشی


1 دانش‌آموخته کارشناسی‌ارشد، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه بوعلی‌سینا، همدان

2 دانشیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه بوعلی‌سینا، همدان


ساپونین­ های تری­ترپنوئیدی یونجه متابولیت­ های ثانویه ­ای هستند که باعث تلخی گیاه، پایین آمدن خوش خوراکی، مقاوم شدن گیاه به حمله آفات و بسیاری از بیماری­ ها می­شوند. ساپونین­ ها همانند گلیکوزید­ها بخشی از گروه استروئید را تشکیل می­ دهند. در این پژوهش، تنوع ساپونین در ارقام مختلفی از  گیاه یونجه که مقاومت­ های متفاوتی در ژرم­ پلاسم مزرعه نسبت به سرخرطومی برگ یونجه نشان داده بودند، از طریق کروماتوگرافی لایه نازک بررسی و مورد آنالیز واقع شد. ساپونین های برگی ارقام تبریز 8 به­ عنوان رقم مقاوم، دیوپوی به ­عنوان رقم حساس و کالیورد 321588 به ­عنوان رقم نیمه ­مقاوم در مرحله آغاز غنچه­ دهی با حلال متانولی مورد عمل استخراج قرار گرفتند. آنالیز ساپونین­ ها روی پلیت­ های کروماتوگرافی نشان داد که در الگوهای نواری، تفاوت­ های کیفی وجود دارد. تعداد باند ساپونینی در رقم مقاوم به سرخرطومی برگ یونجه (تبریز8) 11باند، رقم کالیورد 321588 (نیمه مقاوم به آفت) 8 باند و رقم دیوپوی(حساس به آفت) 6 باند مشاهده گردید. از این پژوهش چنان نتیجه می­ شود که هرچه تعداد باند­های موجود ساپونینی ایجاد شده در ابتدای ژل بیشتر باشد، آن رقم نسبت به آفت مقاوم ­تر است.



عنوان مقاله [English]

The Study of Saponin Profile in 3 Cultivars of Alfalfa (Medicago sativa L.) Expressing Different Levels of Resistance to Alfalfa Weevil (Hypera postica Gyll.) Using TLC

نویسندگان [English]

  • Sahar Bakhtiarian 1
  • Hojatollah Mazahery-Laghab 2
1 M.Sc. Graduate, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan
2 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan
چکیده [English]

Triterpenoid saponins from alfalfa are secondary metabolites which cause plant bittering, decreasing palatability, and increasing the resistance to pests and diseases. Saponins such as glycoside form part of a group of steroid. In present work, the variability of saponins in different cultivars of alfalfa which had presented different levels of resistance towards alfalfa weevil in alfalfa field of germplasm, was studied and analyzed using thin layer chromatography (TLC). Leaf saponins were extracted in methanol solvent from cultivars Tabriz8 as resistant, Deiopoy as susceptible and Kaliverd321588 as semi resistant cultivars before onset of budding stage. The analysis of saponins on TLC plates showed that there are qualitative differences between gel banding patterns. The number of saponins bands in resistant cultivar of alfalfa (Tabriz 8) was 11, in semi resistant (Kaliverd321588) was 8 and in susceptible cultivar (Deiupoy) was observed to be produced 6 bands.  In present work, from the results, As a result, it is concluded that, the findings of the present study more numbers of band spots formed at the origin of sample running on the gel, confirms more resistance to the pest in related cultivar.

کلیدواژه‌ها [English]

  • Resistance to alfalfa weevil
  • Alfalfa cultivars
  • Saponin variability
  • Thin layer chromatography
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