ارزیابی تنوع ژنتیکی ژرم‌پلاسم خاکشیر تلخ با استفاده از نشانگر ISSR

نوع مقاله: علمی - پژوهشی

نویسندگان

1 استادیار گروه بیوتکنولوژی، دانشکده کشاورزی، دانشگاه بوعلی‌سینا، همدان

2 دانشجوی کارشناسی ارشد، گروه بیوتکنولوژی، دانشکده کشاورزی، دانشگاه بوعلی‌سینا، همدان

چکیده

گیاه خاکشیر تلخ، دارای خواص دارویی زیادی می­باشد. مدیریت تنوع ژنتیکی و گیاهان بومی از برنامه ­های حفاظتی ضروری و مهم هر کشوری است. در این تحقیق، تنوع ژنتیکی 33 توده از خاکشیر تلخ با نشانگر مولکولیInter Simple Sequence Repeat (ISSR)  مورد بررسی قرار گرفت. در مجموع 183 مکان کروموزومی ارزیابی شده و در مجموع 1537 باند ایجاد شده و امتیازدهی شدند. 11 آغازگر ISSR مورد استفاده، 100درصد چند­شکلی را نشان دادند. میانگین محتوای اطلاعات چند­شکلی (PIC) 31/5 درصد به‌دست آمد. کمترین مقدار PIC برای آغازگر (AG)8T برابر با مقدار 24/5 درصد و بیشترین مقدار هم برای آغازگر GGGC(GA)8 با مقدار 38/5 بود. حداکثر شاخص نشانگری در آغازگر (AG)8YT برابر با مقدار 6/93 بود بنابراین به‌عنوان آغازگر برتر برای مطالعات بعدی روی انواع خاکشیر معرفی می­شود. الگوی کلی از توزیع تنوع مولکولی برای نشانگرها نشان داد که 17 درصد تنوع مربوط به بین گونه‌ها و 83 درصد تنوع مربوط به درون گونه­ ها می‌باشد. به‌طورکلی می‌توان گفت که تنوع ژنتیکی درون گروه ­ها و گروه ­بندی کردن نمونه ­ها در نقطه برش، با فاصله جغرافیایی که بین استان‌­های مورد مطالعه وجود دارد، هم­خوانی دقیقی ندارد. ازجمله دلایل این پدیده می‌تواند انتقال ژرم­ پلاسم‌ها بین این مکان‌ها و یا اختلاط بذور یا جریان ژنی بین جمعیت‌ها در هنگام برداشت برای مصارف خوراکی و دارویی و خاستگاه مشترک نمونه‌ها باشد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Evaluation of Genetic Diversity in Sisymbrium irio Germplasm Using ISSR Marker

نویسندگان [English]

  • Hedayat Bagheri 1
  • Adel Jafari 2
1 Assistant Professor, Department Biotechnology, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan
2 M.Sc. Student, Department Biotechnology, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan
چکیده [English]

Sisymbrium irio or bitter flixweed is important among the medicinal plants. The human population overgrowth and absence of a comprehensive management of natural resources caused a fast decrease in most valuable indigenous germplasm. Management of genetic diversity of native plants is important in conservation programs. Using molecular techniques, especially ISSR, one can study genetic diversity and analyze the genetic variation. In this study the genetic diversity of 33 populations of S. irio has been investigated by Inter Simple Sequence Repeat (ISSR) marker. In total, 183 sites were investigated. 11 ISSR primers in this study showed 100% polymorphism. The average polymorphism information content (PIC) was equal to 31.5. The minimum amount of PIC for primer (AG) 8T was equal to 24.5 and maximum amount of PIC for primer GGGC (GA) 8 was equal to 038/5. The maximum amount of MI for primer (AG) 8YT was equal to 21.82, therefore it is the most proper primer for further studies. Results showed high variation among and between genotypes and there is the chance of selection and modification.

کلیدواژه‌ها [English]

  • Molecular markers
  • Principal coordinate analysis
  • Marker indicator
  • Polymorphism information content
  • Analysis of molecular variance
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