مقایسه دو روش باززایی مستقیم و غیرمستقیم در تولید برگ‌های حاوی پایه جوانه در چغندرقند

نوع مقاله: علمی - پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی‌ارشد اصلاح نباتات، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد

2 دانشیار گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد

چکیده

چغندرقند یکی از گیاهان صنعتی مهم در ایران و جهان است. پایه جوانه روی برگ، از جمله مهم‌ترین ریزنمونه ­های مورد استفاده در تراریزش این گیاه است. به­ منظور مطالعه ­ی صفت برگ­ های حاوی پایه جوانه در محیط پایه موراشیک و اسکوگ  (MS)به دو روش باززایی مستقیم و غیرمستقیم، یک آزمایش فاکتوریل در قالب طرح کاملاً تصادفی با 4 تکرار انجام گرفت. در روش باززایی مستقیم هورمون­ های بنزیل آدنین (BA) و نفتالین استیک اسید (NAA) هر یک به میزان 5/0 میلی­ گرم در لیتر و در روش باززایی غیرمستقیم هورمون­ های 2-4-D و کینتین  (Kin)هر یک به میزان 0/5میلی ­گرم در لیتر، دو لاین چغندرقند SBSI-04) و (SBSI-02 و سه نوع ریزنمونه­ی برگ پایه جوانه (جوانه انتهایی)، برگ و دمبرگ فاکتورهای مورد مقایسه در این آزمایش بودند. نتایج تجزیه واریانس این مطالعه نشان داد که لاین SBSI-02 با تفاوت معنی­ داری بیشترین باززایی را نشان داد. همچنین اثر نوع ریزنمونه، لاین و روش باززایی روی کارآیی باززایی ریزنمونه ­ها معنی ­داری  (P≤0.01)بود. میزان کارآیی باززایی نیز تحت تأثیر اثرات معنی­ دار (P≤0.01) ریزنمونه × لاین قرار گرفت. نتایج این آزمایش نشان داد روش باززایی اثر معنی ­دار (P≤0.01) روی کارآیی باززایی داشت، اما بین دو روش باززایی مستقیم و غیرمستقیم اختلاف معنی ­داری وجود  (P≤0.01)نداشت.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Comparison of Direct and Indirect Regeneration Methods in Producing Leaf Buds in Sugar Beet

نویسندگان [English]

  • Reza Sharafi 1
  • Farhad Nazarian Firouzabadi 2
  • Ahmad Ismaili 2
1 M.Sc. Student of Plant Breeding, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad
2 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad
چکیده [English]

Sugar beet is an industrial important crop plant in Iran and the rest of the world. Leaf buds emerging on leaves are the most important explants used in the transformation of sugar beet. In order to study the production of leaf buds by direct and indirect organogenesis in sugar beet, an experiment was carried out as factorial in a randomized complete design with four replications. In direct regeneration method, Benzyl adenine (BA, 0.5 mgL-1) and Naphthalene Acetic Acid (NAA, 0.5 mgL-1), and in indirect method 2-4-D (0.5 mgL-1) and kinetin (Kin, 0.5 mgL-1) along with two sugar beet lines (SBSI-04 and SBSI-02), three types of leaf explants, including terminal bud, leaf and petiole, were used. Analysis of variance (Anova) showed that SBSI-02 line produced the highest rate of regeneration. Furthermore, the effect of explant type, line and regeneration method on plantlet regeneration was significant (P ≤ 0.01). Regeneration efficiency was also influenced by the line× explants interaction (P ≤ 0.01). The results of this experiment showed that although the regeneration method had a significant effect on the efficiency of regeneration, but there was no significant difference between direct and indirect regeneration methods.

کلیدواژه‌ها [English]

  • Direct regeneration
  • Efficiency of regeneration
  • Indirect regeneration
  • Shoot tip
  • Sugar beet
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