شناسایی گونه‌های فوزاریوم بیماری‌زا جدا شده از غده‌های سیب‌زمینی در استان کردستان

نوع مقاله : علمی - پژوهشی

نویسندگان

1 استادیار گروه زیست شناسی، دانشکده علوم، دانشگاه رازی کرمانشاه، کرمانشاه

2 دانشجوی کارشناسی‌ارشد میکروبیولوژی دانشگاه آزاد اسلامی واحد علوم تحقیقات کردستان، سنندج

چکیده

گونه‌های فوزاریوم ازجمله قارچ‎های بیماری‎زا در دنیا و ایران هستند که بر روی سیب­ زمینی ایجاد پوسیدگی خشک می‎کنند. بنابراین هدف از انجام این تحقیق شناسایی گونه‎های بیماری­ زای فوزاریوم بر روی سیب­زمینی در سطح مغازه‎های تره­ بار فروشی موجود در شهرهای مختلف استان کردستان بود. در این تحقیق 55 جدایه قارچ فوزاریوم جدا شده از غده‎های آلوده سیب‎زمینی مورد بررسی قرار گرفت که براساس بررسی‎های ریخت شناسی متعلق به گونه‎های Fusarium incarnatum، F. oxysporum،F. acuminatum ،F. graminearum و F. solani بودند. آزمون بیماری­ زایی همه جدایه‌ها بر روی غده‎های سیب­ زمینی سالم (رقم مارفونا) ارزیابی شد. از میان جدایه‎هایی که بیماری­ زا بودند، ده جدایه برای مطالعات مولکولی انتخاب شدند. بررسی­ های مولکولی براساس مقایسه توالی ژن TEF-1α انجام گردید. بر این اساس همه ده جدایه در پنج گروه فیلوژنیتیکی قرار گرفتند. در این تحقیق گونه F. incarnatum برای اولین بار از ایران گزارش‎ و بیماری­ زایی گونه F. graminearum بر روی سیب ­زمینی برای اولین بار از ایران گزارش می‎شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Identification of Pathogenic Fusarium Species Isolated from Potato Tubers in Kurdistan Province

نویسندگان [English]

  • Khosrow Chehri 1
  • Mozhgan Habibi 2
1 Assistant Professor, Department of Biology, Faculty of Sciences, University of Razi, Kermanshah
2 M.Sc. Student of Microbiology, Science and Research Branch, Islamic Azad University of Kurdistan, Sanandaj
چکیده [English]

Members of Fusarium species are common pathogens of potato, causing dry rot in Iran and entire the world. Therefore, the objectives in this study were to isolate and identify disease-causing Fusarium spp. from infected potato tubers in Kurdistan province wet markets based on the morphological and molecular characteristics. Fifty-five isolates of Fusarium were obtained from potato tubers collected from the wet market in different cities of Kurdistan province in Iran. All isolates belonged to F. incarnatum, F. oxysporum, F. acuminatum, F. graminearum, and F. solani through morphological characters. All of the isolates were evaluated for their pathogenicity on healthy potato tubers in the planthouse. The tubers rot symptoms were observed on the 21th day after inoculation of Fusarium isolates on the tubers tested. In the tubers inoculation tests, lesion sizes were quite variable. Based on the sequence data from translation elongation factor (EF-lα) gene analysis, 10 pathogenic Fusarium isolates were divided into five major groups. Although the data obtained from morphological and molecular studies sufficiently supported each other, the resulting phylogenetic trees based on TEF-1α dataset clearly distinguished closely related species and distinctly separated all morphological taxa. This is the first report on molecular identification of Fusarium spp. strains isolated from potato tubers in Iran and F. incarnatum was reported for the first time in Iran and F. graminearum was isolated for the first time from potato tubers inIran.

کلیدواژه‌ها [English]

  • Kurdistan
  • Potato
  • Dry rot
  • Fusarium
  • Mycoflora

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دوفصلنامه فن آوری زیستی در کشاورزی(علمی-پژوهشی)
دوره 7، شماره 1
خرداد 1395
صفحه 93-100

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