بررسی اثر ژن‌های Ehd1 و Ehd3 بر زمان خوشه‌دهی در ارقام برنج

نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسیارشد بیوتکنولوژی کشاورزی، گروه زراعت و اصلاح نباتات، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری

2 استادیار گروه علوم و زیست فناوری گیاهی، دانشکده علوم و فناوری زیستی، دانشگاه شهید بهشتی تهران، تهران

چکیده

کنترل زمان گل‌دهی یکی از مهم‌ترین اجزای اثر متقابل بین گیاهان و محیط رشد آن‌ها می‌باشد که نه تنها برای میزان محصول تولیدی بلکه برای کیفیت دانه برنج نیز عامل مهمی به‌­حساب می‌آید. در این تحقیق مطالعات فنوتیپی و مولکولی بر روی 45 رقم برنج محلی و اصلاح شده انجام شد. ابتدا چندشکلی ژن‌های Ehd1 و Ehd3 در بین ارقام و سپس ارتباط این دو ژن با زمان خوشه‌دهی مورد بررسی قرار گرفت. نتایج مطالعات فنوتیپی حاکی از وجود تنوع بیشتر در ارقام محلی نسبت به ارقام اصلاح شده بود. ارقام محلی به‌طور متوسط 8 روز زودرس‌تر از ارقام اصلاح شده بودند و تفاوت زمان خوشه‌دهی آن‌ها معنی‌دار بود. آغازگرهای اختصاصی طراحی شده براساس هم‌ردیف‌سازی چندگانه، توانست چندشکلی مورد انتظار را بین ارقام مختلف آشکار سازد. تجزیه ارتباط در جمعیت مورد مطالعه نشان داد که ژن Ehd1 نسبت به ژن Ehd3 تأثیر بیشتری بر زمان خوشه‌دهی داشت (1/12 در مقابل 4/5 درصد) و اثر افزایشی آن‌ها به‌ترتیب 4/3 و 2/2 روز برآورد شد. با توجه به کمّی بودن صفت مورد مطالعه و اثر کم دو ژن فوق بر زمان خوشه‌دهی، می‌توان نتیجه‌گیری نمود که به‌نژادگر هنگام اصلاح ارقام زودرس باید به نقش سایر ژن‌ها و اثرات متقابل آن‌ها نیز توجه نماید. 

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Assessment of Impact of Ehd1 and Ehd3 Genes on Heading Date in Rice Cultivars

نویسندگان [English]

  • Leila Nayyeripasand 1
  • Asadollah Ahmadikha 2
1 Former M.Sc. Student of Biotechnology, Department of Agronomy and Plant Breeding, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari
2 Assistant Professor, Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, University of Shahid Beheshti, Tehran
چکیده [English]

Flowering time control is one of the most important components of interactions between plants and their growth environment that is a crucial factor not only for yield rate but also for rice grain quality. In this research phenotypic and molecular studies were conducted in 45 local and improved rice cultivars. Firstly polymorphism of Ehd1 and Ehd3 genes between the cultivars was studied and then the association of the two genes with heading date was investigated. Results of phenotypic studies indicated the existence of higher variation of heading date in local cultivars than that of improved cultivars. Local cultivars had on average 8 days earliness relative to improved ones, with being significant difference between their heading dates. Specific primers designed based on multiple alignments could detect polymorphism between different cultivars. Association studies showed that pooled population Ehd1 had a higher association with heading date than Ehd3 (12.1 vs. 5.4%) and their additive effects were estimated, respectively, 3.4 and 2.2 days. Regarding to quantitative nature of the studied trait and minor effects of the two genes on heading date, it can be concluded that when improving early-mature cultivars utilizing the two genes breeders might consider the roll of other genes and their interactions.

کلیدواژه‌ها [English]

  • Association
  • Heading date
  • Rice
  • Candidate genes
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