تأثیر شوری بلندمدت بر فعالیت‌های آنزیم‌های پراکسیداز و ویژگی‌های فیزیولوژیک گیاه چمنشور (Aeluropus littoralis)

نوع مقاله : علمی - پژوهشی

نویسندگان

1 استادیار گروه علوم پایه، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری

2 دانشآموخته کارشناسی ارشد گروه اصلاح نباتات و بیوتکنولوژی، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری

3 استاد پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری

چکیده

تنش شوری، منجر به تنش اکسیداتیو و افزایش رادیکال‌های آزاد اکسیژن می‌شود که برای سلول سمی می‌باشد. گیاهان از چند سیستم آنتی‌اکسیدانتی برای محافظت از سلول‌های خود علیه این سموم استفاده می‌کنند. در این مطالعه اثر تنش بلندمدت شوری بر فعالیت آنزیم‌های آنتی‌اکسیدانی پراکسیداز و آسکوربات پراکسیداز، کلروفیل، نشت غشائی و روابط آب برگ در گیاه هالوفیت چمن‌شور (Aeluropus littoralis) مورد مطالعه قرار گرفت. بدین منظور ریشه و برگ گیاهان تحت 60 روز تنش شوری با غلظت‌های صفر، 250، 450 و 650 میلی‌مولار NaCl قرار گرفتند. نتایج نشان دادند که فعالیت آنزیم‌های پراکسیداز و آسکوربات پراکسیدازها در برگ تمام تیمارها افزایش یافته است. فعالیت آنزیم پراکسیداز در تنش 450 و 650 میلی‌مولار و آنزیم آسکوربات پراکسیداز در 250 و 450 میلی‌مولار در ریشه افزایش داشت. در 650 میلی‌مولار میزان نشت غشایی افزایش و میزان کلروفیل و محتوای آب نسبی (RWC) کاهش پیدا کرد.    

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Long Term Salinity Stress on Peroxidase Enzymes Activities and Physiological Characteristic of Aeluropus littoralis

نویسندگان [English]

  • Fatemeh Moradian 1
  • Mostafa Modarresi 2
  • Ghorbanali Nematzadeh 3
1 Assistant Professor, Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, Sari
2 M.Sc. Graduated, Department of Agronomy and Plant Breeding, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari
3 Professor, Tabarestan Institute of Genetic and Agricultural Biotechnology, Sari Agricultural Sciences and Natural Resources University, Sari
چکیده [English]

Salinity stress generates oxidative stress and the reactive oxygen radicals which is toxic for cell. Plants adapted to develop antioxidant system to protect the cells from these toxins. In this study, the effect of long term salinity stress was studied on antioxidant enzymes activity like peroxidase and ascorbate peroxidase, chlorophyll, membrane leakage and water relation of leaves in halophyte plant Aeluropus littoralis. The leaves and root of growth plant treated with NaCl at different salt concentration (0, 250, 450 and 650 mM) for a period of 60 days. The results indicated enzyme activity of peroxidase and ascorbate peroxidase increased in all concentration of NaCl in leaves. In root, the activity of peroxidase in 450 and 650 mM and in ascorbate peroxidase in 250 and 450 mM of NaCl increased. In 650 mM of treatment the amount of membrane leakage was high while chlorophyll content and relative water content (RWC) were decrease.

کلیدواژه‌ها [English]

  • Aleuropus littoralis
  • Oxidases
  • Salinity stress
  • Chlorophyll
  • Memberane leakage

مراجع

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دوفصلنامه فن آوری زیستی در کشاورزی(علمی-پژوهشی)
دوره 7، شماره 2
آذر 1395
صفحه 69-75

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