بررسی الگوی الکتروفورز دو بعدی ریشه‌ی کلزای تلقیح شده با باکتری Pseudomonas fluorescens FY32 تحت تنش شوری

نوع مقاله: علمی - پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ‌ارشد، گروه بهنژادی و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه تبریز، تبریز

2 دانشیار، گروه بهنژادی و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه تبریز، تبریز

3 دانشیار، گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه شهید مدنی آذربایجان، تبریز

چکیده

کلزا که به‌منظور استحصال روغن آن تولید می‌گردد، همانند سایر گیاهان زراعی متأثر از شوری می‌شود. شوری خاک به‌عنوان یکی از مهمترین عوامل نامساعد و تنش‌زای غیرزنده اثر نامطلوب بر عملکرد و کیفیت محصولات کشاورزی دارد. باکتریPseudomonasfluorescens جزو باکتری‌های محرک رشد گیاه می‌باشد و در همیاری با گیاهان باعث افزایش رشد مقاومت گیاه در برابر عوامل بیماری‌زا و محدود‌کننده محیطی می­گردد. در این تحقیق شوری در دو سطح صفر و 300 میلی‌مولار نمک NaCl اعمال شد. حضور یا عدم حضور باکتری به‌عنوان تیمارهای دیگر با سه تکرار اعمال گردید و برای تجزیه‌های آماری از طرح کرت‌های خرد شده با طرح پایه‌‌ی کاملاً تصادفی استفاده شد. به‌منظور بررسی پروتئین‌های دخیل در بهبود مقاومت گیاه کلزا به شوری در همیاری با باکتری Pseudomonas fluorescens الگوی الکتروفورز دو بعدی پروتئوم ریشه آن مورد بررسی قرار گرفت. نتایج حاصل نشان داد که از 216 لکه‌ی ظاهر شده 16 لکه‌ی پروتئینی در اثر اعمال تنش شوری، 13 لکه‌ی پروتئینی در حضور باکتری محرک رشد گیاه و 23 لکه در صورت اعمال هر دو تیمار تغییر بیان نشان می‌دهند. پس از شناسایی احتمالی پروتئین‌های مربوطه با استفاده از معیارهای وزن مولکولی و نقطه‌ی ایزوالکتریک مشخص شد این پروتئین‌ها که با افزایش یا کاهش بیان همراه بوده‌اند جزو پروتئین‌های دخیل در مسیرهای متابولیکی/ انرژی‌زایی، پیام‌رسانی، حفاظت و دفاع سلولی، کانالی، ساختار و مکان‌یابی پروتئین‌های دیگر، ساختمان سلولی و رونویسی و ترجمه هستند. با اعمال تیمار شوری یا باکتری یا شوری به‌همراه باکتری، بیشترین تعداد لکه‌های تغییر بیان یافته مربوط به گروه پروتئین‌های متابولیکی/ انرژی‌زایی بود.

کلیدواژه‌ها


عنوان مقاله [English]

Proteome Analysis of Canola Root Inoculated with Pseudomonas fluorescens FY32 under Salinity Stress

نویسندگان [English]

  • Pouya Motie Noparvar 1
  • Ali Bandehagh 2
  • Davood Farajzadeh 3
  • Ebrahim Dorani Uliaie 2
1 M.Sc. Graduated, Department of Plant Biotechnology and Breeding, Faculty of Agriculture, University of Tabriz, Tabriz
2 Associate Professor, Department of Plant Biotechnology and Breeding, Faculty of Agriculture, University of Tabriz, Tabriz
3 Associate Professor, Department of Biology, Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz
چکیده [English]

Canola which is being used for its oil is affected by salinity stress. Salinity is one of the most important abiotic stressful conditions which affects productivity and quality of agricultural products. Pseudomonas fluorescens is a Plant growth promoting rhizobacteria having symbiotic relation with plants that reduces adverse effects and increases plant growth. In this study salinity stress has been applied in two levels (0 and 300 mM NaCl). Absence and presence of P. fluorescens was the other treatment applied to plant root with three replications. Statistical analysis was carried out as split plot based on completely randomized design. In order to evaluate proteome analysis of canola root under salinity stress inoculated with P. fluorescens 2D-PAGE approach was carried out. To reach this goal proteome extract of root was extracted via TCA-acetone method. Isoelectric focusing and SDS-PAGE for 1st and 2nd dimensions, were used, respectively. Acquired gels were analyzed using PD-quest software after staining with silver nitrate. Results demonstrated 216 spots on the gels. Sixteen spots showed significant change as result of salinity stress. Thirteen spots showed change in quantity as result of presence of P. fluorescens FY32. After applying salinity and inoculation PGPR, 23 spots presented changes significantly. After identification of proteins using molecular weight and PI, it has been recognized that most of the responding proteins to salinity and PGPR presence conditions are categorized in metabolic/ energy related pathways. Then, signaling, maintenance/ defense, channel proteins and protein structure/ location related proteins were the ones that had high frequency respectively.

کلیدواژه‌ها [English]

  • 2D-PAGE
  • PGPR
  • Proteome analysis
  • root
  • Canola

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