پاسخ آنتی‌اکسیدانی لیموترش نسبت به باکتری Xanthomonas citri subsp. citri

نوع مقاله: علمی - پژوهشی

نویسندگان

1 دانشجوی دکتری گروه بیماری‌شناسی گیاهی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد

2 دانشیار گروه بیماری‌شناسی گیاهی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد

3 استادیار بخش گیاهپزشکی، مرکز تحقیقات مرکبات کشور، رامسر

چکیده

لیموترش (Citrus aurantifolia) یکی از ژنوتیپ‌های حساس مرکبات نسبت به باکتری Xanthomonas citri subsp. citri می‌باشد که در مناطق جنوب و جنوب غربی ایران کشت داده می‌شود. در این بیماری همه اندام‌های هوایی لیموترش آلوده می‌شوند و در آلودگی‌های شدید سبب برگ‌ریزی، بدشکلی و ریزش میوه، خشکیدگی سرشاخه و ضعف عمومی درخت می‌شود. در این پژوهش، اثر مایه‌زنی مصنوعی برگ‌های لیمو ترش با باکتری Xcc بر فعالیت آنزیم‌های آنتی‌اکسیدانی شامل سوپراکسید دسموتاز، کاتالاز، گایاکول پراکسیداز و آسکوربات پراکسیداز بررسی گردید. در مطالعه ما، فعالیت آنزیم کاتالاز در برگ‌های مایه‌زنی شده به‌طور معنی‌داری بیشتر از برگ‌های شاهد بود. هم‌چنین نتایج فعالیت آنزیم گایاکول پراکسیداز نشان داد که فعالیت آن به‌طور معنی‌داری در بازه زمانی دو، پنج و هفت روز بعد از مایه‌زنی افزایش یافته است. در فعالیت آنزیم سوپراکسید دسموتاز در برگ‌های آلوده تغییر معنی‌داری مشاهده نشد. هم‌چنین فعالیت آنزیم آسکوربات پراکسیداز در برگ‌های مایه‌زنی شده در طول مدت آلودگی با باکتری Xcc بیشتر از شاهد بود، اما فقط در بازه زمانی دو و 10 روز معنی‌دار بود. نتایج حاصل از این تحقیق می‌تواند روش‌های جدیدی را در تحقیقات آینده به‌منظور کنترل شانکر، از طریق دستکاری در سازوکارهای دفاعی گیاه فراهم شود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Antioxidant Response of Mexican Lime to Xanthomonas citri subsp. citri

نویسندگان [English]

  • Hossein Mirzaei-Najafgholi 1
  • Saeid Tarighi 2
  • Morteza Golmohammadi 3
  • Parisa Taheri 2
1 PhD Student, Department of Plant Pathology, Faculty of Agriculture, Ferdowsi Uinversity of Mashhad, Mashhad
2 Associate Professor, Department of Plant Pathology, Faculty of Agriculture, Ferdowsi Uinversity of Mashhad, Mashhad
3 Associate Professor, Department of Plant Protection, Iran Citrus Research Institute, Ramsar, Iran
چکیده [English]

Mexican lime(Citrus aurantifolia) is one of the susceptible citrus genotype to Xanthomonas citri subsp. citri (Xcc) which is grown in the south and south-west of Iran. The disease infects all aerial parts of Mexican lime and severely infected plants show defoliation, badly fruit, premature fruit drop, twig dieback and general tree decline. In this study, Mexican lime leaves were artificially inoculated with Xcc to determine the effect of Xcc infections on antioxidant enzymes activity such as superoxide dismutase (SOD), guaiacol peroxidase (GP), catalase (CAT) and ascorbate peroxidase (APOD). These results indicated that CAT activity was significantly higher in infected leaves than controls. No effect on SOD activity was observed in infected leaves. The result also showed that POD was significantly increased at 2, 5 and 7 days post inoculation. While, APOD activity was higher than control in inoculated leaves during infected period with Xcc, considering that the results was significant only in 2 and 10 days post inoculation. Current results can present new approaches in the future researches in order to control CBC via transformation of plant defense.

کلیدواژه‌ها [English]

  • Catalase
  • Superoxide dismutase
  • Guaiacol peroxidase
  • Ascorbate peroxidase

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