شناسایی جایگاه صفات کمی تأثیرگذار بر صفات اسکلتی بر روی کروموزوم دوم بلدرچین ژاپنی

نوع مقاله: علمی - پژوهشی

نویسندگان

1 دانش‌آموخته دکتری گروه علوم دامی، دانشکده علوم کشاورزی و صنایع غذایی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران، تهران، ایران

2 استاد، گروه علوم دامی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان

3 دانش‌آموخته کارشناسی‌ارشد، گروه علوم دامی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان

چکیده

هدف از انجام این تحقیق شناسایی جایگاه‌های صفات کمی مرتبط با صفات اسکلتی روی کروموزوم شماره دو بلدرچین ژاپنی بود. تعداد هشت پرنده نر و هشت پرنده ماده از دو سویه وحشی (W) و سفید (S) به عنوان نسل والد (F0) انتخاب و بین آن‌ها تلاقی دوطرفه صورت گرفت. تعداد 422 پرنده نسل F2 حاصل از تلاقی تصادفی پرندگان نسل F1 (17 نتاج (SW) حاصل از تلاقی نر سفید × ماده وحشی (14 پرنده ماده و سه پرنده نر) و 17 نتاج (WS) حاصل از تلاقی نر وحشی × ماده سفید (11 پرنده ماده و شش پرنده نر)) در پایان دوره 35 روزه پرورش کشتار شده و مورد رکورد برداری فنوتیپی صفات مربوط به استخوان قرار گرفته و نمونه‌های خون آن‌ها برای تعیین ژنوتیپ چهار نشانگر ریزماهواره روی کروموزوم دو جمع‌آوری شدند. آنالیز QTL به روش مکان‌یابی درون فاصله‌ای مبتنی بر رگرسیون انجام گردید و اثر عوامل QTL با استفاده از سه مدل آماری متفاوت (مدل افزایشی، مدل افزایشی و غلبه، مدل افزایشی، غلبه و نقش‌پذیری) برآورد و 19 QTL مرتبط با نه صفت شناسایی شد. صفات وزن پای چپ، وزن ران و قطر پای چپ و راست تقریباً در بیشتر مدل‌ها دارای QTL معنی‌دار بودند. نتایج این مطالعه نشان داد که جایگاه‌های ژنی مؤثری روی کروموزوم شماره دو وجود دارد که با داشتن اثر عوامل پلیوتروپی و وراثت غیرمندلی (نقش‌پذیـری ژنومی) بر روی برخی از صـفات استخوانی با سطح معـنی‌داری P < 0.01) و  (P < 0.05 تأثیر می‌گذارند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Identification of QTL (Quantitative Trait Loci) Affecting Skeletal Traits on Second Chromosome of Japanese Quail

نویسندگان [English]

  • Ehsan Nasirifar 1
  • Ali K. Esmailizadeh 2
  • Marjan Ahmadi 3
1 PhD Graduated, Department of Animal Sciences, Faculty of Agricultural Sciences and Food Industries, Islamic Azad University, Science and Research Branch, Tehran, Iran
2 Professor, Department of Animal Sciences, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman
3 MSc Graduated, Department of Animal Sciences, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman
چکیده [English]

This study aims to identify quantitative trait loci (QTL) related to the skeletal traits of Japanese quail on chromosome 2. Eight males and eight females from wild type (W) and white (S)) were selected as the parent generation (F0) and the crossing between them was reciprocal. The population consisted 422 birds of F2 population through random mating of F1 generation (17 progenies (SW) derived from crosses between white male × wild female (14 females and three males) and 17 progenies (WS) derived from crosses between wild male × white female (11 females and six males)), which were slaughtered at the end of a 35-day period of breeding. Subsequently, the phenotypic characteristics of the bone were recorded and blood samples were taken for genotyping four microsatellite markers on chromosome 2. QTL analysis was performed by least squares interval mapping method based on regression; QTL effects were estimated using three different statistical models (additive model; additive and dominance model; additive, dominance, and imprinting model); and 19 QTL were identified with nine traits. The weight of left foot, femur weight, and diameter of left and right feet had significant QTL almost in most of the models. The results of this study showed that there were significant loci on chromosome 2, which could affect some traits of bone at the significance level of P

کلیدواژه‌ها [English]

  • QTL analysis
  • Polytropic
  • Microsatellite markers
  • Imprinting
  • Mapping

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