نوع مقاله : مقاله پژوهشی
نویسنده
استادیار موسسه تحقیقات اصلاح و تهیه نهال و بذر (SPII)، سازمان آموزش، تحقیق و ترویج کشاورزی (AREEO)
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسنده [English]
Red cabbage is one of the most important members of the Brassicaceae family, which its hybrids are cultivated globally. The production of inbred lines is a prerequisite for hybrid breeding programs and genetic studies in this cross-pollinated species which their production via conventional approaches tend to be time-consuming. Although shortening the breeding processes through the isolated microspore culture technique has been optimized to some extent in Brassicaceae, the efficiency of this process in red cabbage is still low. In this research, the effects of epigenetic modifiers, including trichostatin A (at concentrations of 0, 0.1, 0.2, 0.5, and 1.0 μM for 30 and 60 min) and 5-azacytidine (at concentrations of 0, 1.25, 2.5, 5, and 10 μM for 48 and 96 hours), were investigated to optimize the survival and subsequent embryogenesis of red cabbage microspores. Trichostatin A at concentrations of 0.2 and 0.5 μM for 30 min and also 0.2 μM for 60 min increased embryogenesis by 2.9-3.1 times, compared to the control. In contrast, treatment with 5-azacytidine at concentrations of 1.25 and 2.5 μM did not have a significant impact on embryogenesis efficiency at probability level of 5%. Higher concentrations of 5-azacytidine exerted negative effect on viability and embryogenesis so that the least number of embryos were observed at concentrations of 5 and 10 μM. The results of this study indicated that the optimal concentration of trichostatin A is associated with increased microspore survival post-stress, leading to enhanced production of haploid embryos.
کلیدواژهها [English]
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