واکنش زنجیره ای پلیمراز جهت بررسی پتانسیل ژنتیکی تولید داکسی‌نیوالنول و نیوالنول در جدایه‌های قارچ Fusarium culmorum گندم در استان آذربایجان غربی

نوع مقاله: علمی - پژوهشی

نویسندگان

1 فارغ التحصیل کارشناسی ارشد بیماری شناسی گیاهی، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه زابل، زابل

2 دانشیار گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه زابل، زابل

3 استادیار گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه زابل، زابل

4 مربی گروه شیمی دارویی، دانشکده داروسازی، دانشگاه علوم پزشکی زابل، زابل

چکیده

بلایــت فوزاریومی سنبــــله یکـی از مهـم­ترین بیماری­های غلات در جهان به­شــمار می­آید. قـــارچ­های Fusarium culmorum و  F. graminearum مهم­ترین عوامل ایجادکننده این بیماری می­باشند. این قارچ­ها دانه­ها را توسط مایکوتوکسین­ها آلوده نموده و منجر به کاهش کمی و کیفی محصول می­گردند. در این بررسی تعداد 98 جدایه F. culmorum جداسازی شده از مــزارع اســتان آذربایجان­غربی، با استفاده از کلیدهای شناسایی و جفت آغازگر اختصاصی گونه C51F/C51R شناسایی گردیدند. با استفاده از واکنش زنجیره­ای پلیمراز، توانایی تولید نیوالنول (NIV) و داکسی­نیوالنول (DON) توسط جدایه­های  F. culmorum مورد ارزیابی قرار گرفت. ژن Tri7 با استفاده از جفت آغازگرهای Tri7F/Tri7R و MinusTri7R/MinusTri7F مورد ردیابی قرار گرفت. با استناد به نتایج حاصل از واکنش زنجیره­ای پلیمراز، تعداد 59 جدایه به­عنوان جدایه­های دارای پتانسیل تولید تیپ شیمیایی DON و 39 جدایه به­عنوان جدایه­های دارای پتانسیل تولید تیپ شیمیایی NIV معرفی گردیدند. با توجه به یافته­های این تحقیق تیپ شیمیایی DON به­عنوان تیپ شیمیایی غالب شناسایی گردید. در این تحقیق برای اولین­بار در کشور، تیپ­های شیمیایی تولید شده توسط قارچ F. culmorum مورد بررسی قرار گرفته است.

کلیدواژه‌ها


عنوان مقاله [English]

PCR Reaction for Surveying the Genetic Potential of Deoxynivalenol and Nivalenol Production of the Wheat usarium culmorum Isolates in the West Azarbayjan Province

نویسندگان [English]

  • azad lava 1
  • Mohammad salari 2
  • seyed kazem sabagh 3
  • saeedeh sepeheri kiya 4
چکیده [English]

Fusarium Head Blight is known as one of the most important cereal diseases in the world. Fusarium culmorum and F. graminearum are the major causal agents of this disease throughout the world. F. culmorum and F. graminearum contaminate seeds with mycotoxins and decreases the product quality and quantity. In this investigate, a total of 98 isolates from different fields of west Azarbayjan province, Iran, were identified as F. culmorum using traditional identification techniques and C51F/C51R species-specific primer pair. In order to assess the potential of isolates to produce nivalenol (NIV) and deoxynivalenol (DON), we used PCR assay. Tri7 gene was detected by primer pairs Tri7F/Tri7R and MinusTri7F/MinusTri7R. Out of the 98 tested F. culmorum isolates with Tri7 PCR assays, 59 isolates were introduced as DON producers and 39 isolates introduced as NIV producers. According to our findings, DON chemotype is predominating in west Azarbayjan province. To the best of our knowledge, Chemotyping of F. culmorum isolates is reported for the first time from Iran.

کلیدواژه‌ها [English]

  • Chemotype
  • Trichothecene
  • PCR
  • Tri7
  • West Azarbayjan
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